TMX3 Polyclonal antibody

TMX3 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 21040-1-AP

Publications(3)

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

EC:5.3.4.1, PDIA13, Protein disulfide-isomerase TMX3, Thioredoxin domain-containing protein 10, Thioredoxin-related transmembrane protein 3

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

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Filter: AllWBIHC

WB analysis of mouse brain using 21040-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 21040-1-AP (TMX3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse brain using 21040-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21040-1-AP (TMX3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse brain using 21040-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21040-1-AP (TMX3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human testis using 21040-1-AP
Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 21040-1-AP (TMX3 Antibody) at dilution of 1:50 (under 10x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human testis using 21040-1-AP
Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 21040-1-AP (TMX3 Antibody) at dilution of 1:50 (under 40x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human stomach using 21040-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 21040-1-AP (TMX3 Antibody) at dilution of 1:50 (under 10x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human stomach using 21040-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 21040-1-AP (TMX3 Antibody) at dilution of 1:50 (under 40x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Overview

Tested Applications
Product Information
Publications (3)
Protocols

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	mouse brain tissue
Positive IHC detected in	mouse brain tissue, human stomach tissue, human testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:200-1:1000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21040-1-AP targets TMX3 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag15376 Product name: Recombinant human TMX3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-191 aa of BC032325 Sequence: MAAWKSWTALRLCATVVVLDMVVCKGFVEDLDESFKENRNDDIWLVDFYAPWCGHCKKLESIWNEVGLEMKSIGSPVKVGKMDATSYSSIASEFGVRGYPTIKLLKGDLAYNYRGPRTKDDIIEFAHRVSGALIRPLPSQQMFEHMQKRHRVFFVYVGGESPLKEKYIDAASELIVYTYFFSASEEVVPEV Predict reactive species
Full Name	thioredoxin-related transmembrane protein 3
Calculated Molecular Weight	454 aa, 52 kDa
Observed Molecular Weight	65 kDa
GenBank Accession Number	BC032325
Gene Symbol	TMX3
Gene ID (NCBI)	54495
RRID	AB_2878798
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q96JJ7
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IHC protocol for TMX3 antibody 21040-1-AP	Download protocol
WB protocol for TMX3 antibody 21040-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

Species	Application	Title
human, mouse	WB	bioRxiv SLC33A1 exports oxidized glutathione to maintain endoplasmic reticulum redox homeostasis. Authors - Shanshan Liu View Article
human,mouse	WB	Nat Cell Biol SLC33A1 exports oxidized glutathione to maintain endoplasmic reticulum redox homeostasis. Authors - Shanshan Liu View Article
	WB	Autophagy The ER protein CANX (calnexin)-mediated autophagy protects against alzheimer disease Authors - Hongtao Shen View Article