Tested Applications
| Positive WB detected in | HepG2 cells, mouse liver tissue |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 4 publications below |
| IF | See 2 publications below |
Product Information
15459-1-AP targets TNIP2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag7731 Product name: Recombinant human TNIP2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 76-429 aa of BC002740 Sequence: EQLRRQEGGAAEAQMRQEIERLTERLEEKEREMQQLLSQPQHEREKEVVLLRRSMAEGERARAASDVLCRSLANETHQLRRTLTATAHMCQHLAKCLDERQHAQRNVGERSPDQSEHTDGHTSVQSVIEKLQEENRLLKQKVTHVEDLNAKWQRYNASRDEYVRGLHAQLRGLQIPHEPELMRKEISRLNRQLEEKINDCAEVKQELAASRTARDAALERVQMLEQQILAYKDDFMSERADRERAQSRIQELEEKVASLLHQVSWRQDSREPDAGRIHAGSKTAKYLAADALELMVPGGWRPGTGSQQPEPPAEGGHPGAVQRGQGDLQCPHCLQCFSDEQGEELLRHVAECCQ Predict reactive species |
| Full Name | TNFAIP3 interacting protein 2 |
| Calculated Molecular Weight | 49 kDa |
| Observed Molecular Weight | 50 kDa |
| GenBank Accession Number | BC002740 |
| Gene Symbol | TNIP2 |
| Gene ID (NCBI) | 79155 |
| RRID | AB_10859096 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8NFZ5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TNIP2, also named as ABIN2 and FLIP1, inhibits NF-kappa-B activation by blocking the interaction of RIPK1 with its downstream effector NEMO/IKBKG. It acts as transcriptional coactivator when translocated to the nucleus. TNIP2 has some isoforms with MW 49 kDa and 37 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for TNIP2 antibody 15459-1-AP | Download protocol |
| IHC protocol for TNIP2 antibody 15459-1-AP | Download protocol |
| IP protocol for TNIP2 antibody 15459-1-AP | Download protocol |
| WB protocol for TNIP2 antibody 15459-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Sci Signal The kinase TPL2 activates ERK and p38 signaling to promote neutrophilic inflammation. | ||
J Virol Foot-and-Mouth Disease Virus Structural Protein VP1 Destroys the Stability of TPL2 Trimer by Degradation TPL2 to Evade Host Antiviral Immunity. | ||
Neurosci Lett TNIP2 inhibits amyloidogenesis by regulating the 3'UTR of BACE1: an in vitro study | ||
Neuropeptides Protective effect of TNIP2 on the inflammatory response of microglia after spinal cord injury in rats | ||
Immun Inflamm Dis TNFAIP3 interacting protein 2 relieves lipopolysaccharide (LPS)-induced inflammatory injury in endometritis by inhibiting NF-kappaB activation |
