TNNI1 Polyclonal antibody

TNNI1 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 16102-1-AP

Synonyms

SSTNI, TNN1, TNNI1



Tested Applications

Positive WB detected inmouse skeletal muscle tissue, human skeletal muscle tissue, rat skeletal muscle tissues
Positive IHC detected inhuman skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16102-1-AP targets TNNI1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TNNI1 fusion protein Ag9001
Full Name troponin I type 1 (skeletal, slow)
Calculated Molecular Weight 187 aa, 22 kDa
Observed Molecular Weight 25 kDa
GenBank Accession NumberBC012600
Gene Symbol TNNI1
Gene ID (NCBI) 7135
RRIDAB_2206103
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for TNNI1 antibody 16102-1-APDownload protocol
IHC protocol for TNNI1 antibody 16102-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB,IF

Basic Res Cardiol

Alkaline nucleoplasm facilitates contractile gene expression in the mammalian heart.

Authors - Alzbeta Hulikova
humanWB

iScience

Engineering of thick human functional myocardium via static stretching and electrical stimulation.

Authors - Danielle Pretorius
mouseWB

Sci Rep

A missense mutation in the RSRSP stretch of Rbm20 causes dilated cardiomyopathy and atrial fibrillation in mice.

Authors - Kensuke Ihara
mouseWB

Front Physiol

Lipidomic Analyses Reveal Specific Alterations of Phosphatidylcholine in Dystrophic Mdx Muscle.

Authors - William J Valentine
humanWB

Cell Death Discov

Long noncoding RNA LINC00284 facilitates cell proliferation in papillary thyroid cancer via impairing miR-3127-5p targeted E2F7 suppression.

Authors - Bin Zhou
mouseIF

Front Med

Distinct mononuclear diploid cardiac subpopulation with minimal cell-cell communications persists in embryonic and adult mammalian heart

Authors - Miaomiao Zhu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Xuebin (Verified Customer) (11-12-2018)

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:50
  • Cell Tissue Type: heart