TNNI1 Polyclonal antibody

TNNI1 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Cat No. 16102-1-AP

Publications(8)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Synonyms

SSTNI, TNN1, Troponin I, slow-twitch isoform

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

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Filter: AllWBIHCIF-P

WB analysis of human skeletal muscle using 16102-1-AP
human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 16102-1-AP (TNNI1 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis using 16102-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 16102-1-AP (TNNI1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse skeletal muscle using 16102-1-AP
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 16102-1-AP (TNNI1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse skeletal muscle using 16102-1-AP
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 16102-1-AP (TNNI1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of mouse skeletal muscle using 16102-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skeletal muscle tissue using TNNI1 antibody (16102-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Overview

Tested Applications
Product Information
Publications (8)
Protocols
Reviews (1)

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	mouse skeletal muscle tissue, human skeletal muscle tissue, rat skeletal muscle tissues
Positive IHC detected in	mouse skeletal muscle tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	mouse skeletal muscle tissue

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunohistochemistry (IHC)	IHC : 1:500-1:2000
Immunofluorescence (IF)-P	IF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16102-1-AP targets TNNI1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag9001 Product name: Recombinant human TNNI1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-187 aa of BC012600 Sequence: MPEVERKPKITASRKLLLKSLMLAKAKECWEQEHEEREAEKVRYLAERIPTLQTRGLSLSALQDLCRELHAKVEVVDEERYDIEAKCLHNTREIKDLKLKVMDLRGKFKRPPLRRVRVSADAMLRALLGSKHKVSMDLRANLKSVKKEDTEKERPVEVGDWRKNVEAMSGMEGRKKMFDAAKSPTSQ Predict reactive species
Full Name	troponin I type 1 (skeletal, slow)
Calculated Molecular Weight	187 aa, 22 kDa
Observed Molecular Weight	25 kDa
GenBank Accession Number	BC012600
Gene Symbol	TNNI1
Gene ID (NCBI)	7135
RRID	AB_2206103
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P19237
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IF protocol for TNNI1 antibody 16102-1-AP	Download protocol
IHC protocol for TNNI1 antibody 16102-1-AP	Download protocol
WB protocol for TNNI1 antibody 16102-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

Species	Application	Title
rat	WB,IF	Basic Res Cardiol Alkaline nucleoplasm facilitates contractile gene expression in the mammalian heart. Authors - Alzbeta Hulikova View Article
human	WB	iScience Engineering of thick human functional myocardium via static stretching and electrical stimulation. Authors - Danielle Pretorius View Article
mouse	WB	Sci Rep A missense mutation in the RSRSP stretch of Rbm20 causes dilated cardiomyopathy and atrial fibrillation in mice. Authors - Kensuke Ihara View Article
mouse	WB	Front Physiol Lipidomic Analyses Reveal Specific Alterations of Phosphatidylcholine in Dystrophic Mdx Muscle. Authors - William J Valentine View Article
human	WB	Cell Death Discov Long noncoding RNA LINC00284 facilitates cell proliferation in papillary thyroid cancer via impairing miR-3127-5p targeted E2F7 suppression. Authors - Bin Zhou View Article
		Cell Stem Cell Human PSC-derived sinoatrial node-cardiac plexus assembloids model innervation-associated maturation of pacemaker systems. Authors - Tongdong Zhang View Article