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TNNI2 Polyclonal antibody

TNNI2 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 22130-1-AP

Synonyms

AMCD2B, DA2B, FSSV, fsTnI, TNNI2, Troponin I fast skeletal muscle, Troponin I, fast-twitch isoform

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of mouse skeletal muscle tissue using TNNI2 Polyclonal antibody (22130-1-AP)

    WB analysis of mouse skeletal muscle using 22130-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 22130-1-AP (TNNI2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using TNNI2 Polyclonal antibody (22130-1-AP)

    WB analysis using 22130-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 22130-1-AP (TNNI2 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat skeletal muscle tissue using TNNI2 Polyclonal antibody (22130-1-AP)

    WB analysis of rat skeletal muscle using 22130-1-AP

    rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 22130-1-AP (TNNI2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse skeletal muscle tissue using TNNI2 Polyclonal antibody (22130-1-AP)

    IP experiment of mouse skeletal muscle using 22130-1-AP

    IP result of anti-TNNI2 (IP:22130-1-AP, 4ug; Detection:22130-1-AP 1:500) with mouse skeletal muscle tissue lysate 4000ug.

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using TNNI2 Polyclonal antibody (22130-1-AP)

    IHC staining of human skeletal muscle using 22130-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle slide using 22130-1-AP (TNNI2 Antibody) at dilution of 1:50.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using TNNI2 Polyclonal antibody (22130-1-AP)

    IHC staining of mouse skeletal muscle using 22130-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 22130-1-AP (TNNI2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse skeletal muscle tissue using TNNI2 Polyclonal antibody (22130-1-AP)

    IF Staining of mouse skeletal muscle using 22130-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using TNNI2 antibody (22130-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of mouse skeletal muscle tissue using TNNI2 Polyclonal antibody (22130-1-AP)

    IF Staining of mouse skeletal muscle using 22130-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using TNNI2 antibody (22130-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inmouse skeletal muscle tissue, rat skeletal muscle tissue
Positive IP detected inmouse skeletal muscle tissue
Positive IHC detected inmouse skeletal muscle tissue, human skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse skeletal muscle tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:20000-1:100000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

22130-1-AP targets TNNI2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TNNI2 fusion protein Ag17750
Full Name troponin I type 2 (skeletal, fast)
Calculated Molecular Weight 182 aa, 21 kDa
Observed Molecular Weight 21-26 kDa
GenBank Accession NumberBC032148
Gene Symbol TNNI2
Gene ID (NCBI) 7136
RRIDAB_2879002
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for TNNI2 antibody 22130-1-APDownload protocol
IHC protocol for TNNI2 antibody 22130-1-APDownload protocol
IF protocol for TNNI2 antibody 22130-1-APDownload protocol
IP protocol for TNNI2 antibody 22130-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Ann Surg Oncol

Troponin I2 as a Specific Biomarker for Prediction of Peritoneal Metastasis in Gastric Cancer.

Authors - Koichi Sawaki
View Article
mouseWB

Sci Rep

A missense mutation in the RSRSP stretch of Rbm20 causes dilated cardiomyopathy and atrial fibrillation in mice.

Authors - Kensuke Ihara
View Article
mouseWB

Front Physiol

Lipidomic Analyses Reveal Specific Alterations of Phosphatidylcholine in Dystrophic Mdx Muscle.

Authors - William J Valentine
View Article
mouseWB

J Muscle Res Cell Motil

Differential regulation of Actn2 and Actn3 expression during unfolded protein response in C2C12 myotubes.

Authors - Nagakatsu Harada
View Article