TPD52L2 Antibody
Rabbit Polyclonal
| Tested applications | ELISA, WB, IHC, IF |
| Cited applications | |
| Species specificity | human, mouse, rat; other species not tested. |
| Cited species | |
| Positive WB detected in | HEK-293 cells, HeLa cells, human brain tissue, human kidney tissue, Jurkat cells, K-562 cells, MCF7 cells, mouse brain tissue |
| Positive IHC detected in | human breast cancer |
| Positive IF detected in | HepG2 cells |
| Recommended dilution | WB: 1:200-1:2000 IHC: 1:20-1:200 IF: 1:20-1:50 |
IP = Immunoprecipitation
HEK-293 cells were subjected to SDS PAGE followed by western blot with 11795-1-AP(TPD52L2 Antibody) at dilution of 1:800
Immunohistochemistry of paraffin-embedded human breast cancer using 11795-1-AP(TPD52L2 Antibody) at Dilution 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human breast cancer using 11795-1-AP(TPD52L2 Antibody) at Dilution 1:50 (under 40x lens)
Immunofluorescent analysis of HepG2 cells, using TPD52L2 antibody 11795-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
| Source | Rabbit | Purification method | Antigen affinity purification |
| Isotype | IgG | Storage | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
| Immunogen | TPD52L2 fusion protein ag2364 | Full name | tumor protein D52-like 2 |
|
Calculated molecular weight |
206aa,22kd |
Observed molecular
weight |
25-30kd |
|
GenBank accession number |
BC006804 | Gene ID (NCBI) | 7165 |
| Gene symbol | TPD52L2 | Synonyms | D54; DKFZp686A1765; hD54 |
Tumor protein D52-like proteins (TPD52) are small coiled-coil motif bearing proteins that were first identified in breast carcinoma. Three human TPD52 members had been identified, named hD52 (TPD52), hD53 (TPD52L1), and hD54 (TPD52L2). The most important characteristic of the protein family is a highly conserved coiled-coil motif that is required for homo- and heteromeric interaction with other TPD52-like proteins. TPD52 and related proteins have been implicated in cell proliferation, apoptosis, and vesicle trafficking. TPD52L2 has five isoforms produced by alternative splicing, and its multiple sites have been identified to be phosphorylated. Interaction of TPD52L2 with MAL2, a novel member of the MAL proteolipid family, may be required for the role of TPD52L2 in vesicle transport. |
