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  • KD/KO Validated

TPK1 Polyclonal antibody

TPK1 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10942-1-AP

Synonyms

HTPK1, Placental protein 20, PP20, thiamin pyrophosphokinase 1, Thiamine pyrophosphokinase 1, TPK1



Tested Applications

Positive WB detected inSH-SY5Y cells, HEK-293 cells, human small intestine tissue, mouse testis tissue, L02 cells, mouse small intestine tissue, mouse kidney tissue, rat small intestine tissue
Positive IP detected inmouse kidney tissue
Positive IHC detected inhuman kidney tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10942-1-AP targets TPK1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TPK1 fusion protein Ag1404
Full Name thiamin pyrophosphokinase 1
Calculated Molecular Weight 28 kDa
Observed Molecular Weight 28 kDa
GenBank Accession NumberBC014552
Gene Symbol TPK1
Gene ID (NCBI) 27010
RRIDAB_2205647
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TPK1(Thiamin pyrophosphokinase 1) is also named as PP20(placental protein 20) and belongs to the thiamine pyrophosphokinase family. It is a cellular enzyme involved in the regulation of thiamine metabolism and catalyzes the conversion of thiamine, a form of vitamin B1, to thiamine pyrophosphate (TDP, or TPP). There is no difference in TPK1 expression in cultured fibroblasts from normal subjects or from patients with thiamine-responsive megaloblastic anemia(PMID:11342111). It can exsit as a dimer(PMID:21552434). Defects in TPK1 are the cause of thiamine metabolism dysfunction syndrome type 5, episodic encephalopathy type (THMD5).

Protocols

Product Specific Protocols
WB protocol for TPK1 antibody 10942-1-APDownload protocol
IHC protocol for TPK1 antibody 10942-1-APDownload protocol
IP protocol for TPK1 antibody 10942-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Sci Adv

Dietary thiamine influences l-asparaginase sensitivity in a subset of leukemia cells.

Authors - Rohiverth Guarecuco
  • KO Validated
mouseWB

Proc Natl Acad Sci U S A

OCT1 is a high-capacity thiamine transporter that regulates hepatic steatosis and is a target of metformin.

Authors - Ligong Chen
humanWB

Oncotarget

Identification of vitamin B1 metabolism as a tumor-specific radiosensitizing pathway using a high-throughput colony formation screen.

Authors - Gaganpreet S Tiwana
humanWB

Am J Pathol

Identification of aurora kinase B and Wee1-like protein kinase as downstream targets of (V600E)B-RAF in melanoma.

Authors - Sharma Arati A
ratWB

Sci Rep

Thiamine metabolism is critical for regulating correlated growth of dendrite arbors and neuronal somata.

Authors - Huimin Liu
humanWB

PLoS One

Metabolic effects of acute thiamine depletion are reversed by rapamycin in breast and leukemia cells.

Authors - Shuqian Liu