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TPSD1 Polyclonal antibody

TPSD1 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC,ELISA

Conjugate

Unconjugated

Cat no : 55239-1-AP

Synonyms

Delta tryptase, HmMCP 3 like tryptase III, Mast cell mMCP 7 like, MCP7 LIKE, MCP7L1, MMCP 7L, TPSD1, Tryptase 3, Tryptase delta, tryptase delta 1

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse lung tissue using TPSD1 Polyclonal antibody (55239-1-AP)

    WB analysis of mouse lung using 55239-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 55239-1-AP (TPSD1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of A549 cells using TPSD1 Polyclonal antibody (55239-1-AP)

    IP experiment of A549 using 55239-1-AP

    IP result of anti-TPSD1 (IP:55239-1-AP, 3ug; Detection:55239-1-AP 1:300) with A549 cells lysate 920ug.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using TPSD1 Polyclonal antibody (55239-1-AP)

    IHC staining of human lung cancer using 55239-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 55239-1-AP (TPSD1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using TPSD1 Polyclonal antibody (55239-1-AP)

    IHC staining of human lung cancer using 55239-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 55239-1-AP (TPSD1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inmouse lung tissue
Positive IP detected inA549 cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

55239-1-AP targets TPSD1 in WB, IP, IHC,ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name tryptase delta 1
Calculated Molecular Weight 27 kDa
Observed Molecular Weight 26-30 kDa
GenBank Accession NumberNM_012217
Gene Symbol TPSD1
Gene ID (NCBI) 23430
RRIDAB_2881293
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for TPSD1 antibody 55239-1-APDownload protocol
IHC protocol for TPSD1 antibody 55239-1-APDownload protocol
IP protocol for TPSD1 antibody 55239-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols