Filter: AllWBIHCIF/ICC

WB analysis of mouse brain using 10332-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 10332-1-AP (TRAIP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse placenta using 10332-1-AP
mouse placenta tissue were subjected to SDS PAGE followed by western blot with 10332-1-AP (TRAIP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 10332-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 10332-1-AP (TRAIP antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of human placenta using 10332-1-AP
human placenta tissue were subjected to SDS PAGE followed by western blot with 10332-1-AP (TRAIP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of A549 using 10332-1-AP
A549 cells were subjected to SDS PAGE followed by western blot with 10332-1-AP (TRAIP Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 10332-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 10332-1-AP (TRAIP Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of human testis using 10332-1-AP
Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10332-1-AP (TRAIP Antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human testis using 10332-1-AP
Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10332-1-AP (TRAIP Antibody) at dilution of 1:200 (under 40x lens).
IF Staining of HepG2 using 10332-1-AP
Immunofluorescent analysis of HepG2 cells using 10332-1-AP (TRAIP antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Featured Product
KD/KO Validated

TRAIP Polyclonal antibody

Name: TRAIP Polyclonal antibody
Brand: Proteintech
SKU: 10332-1-AP

TRAIP Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 10332-1-AP

Publications(9)

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

TRIP, TRAF-interacting protein, TRAF interacting protein, RNF206, RING finger protein 206

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

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Overview

Tested Applications
Product Information
Publications (9)
Protocols

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	mouse brain tissue, A549 cells, HepG2 cells, human brain tissue, human placenta tissue, mouse placenta tissue
Positive IHC detected in	human testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 5 publications below
WB	See 8 publications below
IHC	See 5 publications below
IP	See 1 publications below

Product Information

10332-1-AP targets TRAIP in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag0491 Product name: Recombinant human TRAIP protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-204 aa of BC000310 Sequence: MPIRALCTICSDFFDHSRDVAAIHCGHTFHLQCLIQWFETAPSRTCPQCRIQVGKRTIINKLFFDLAQEEENVLDAEFLKNELDNVRAQLSQKDKEKRDSQVIIDTLRDTLEERNATVVSLQQALGKAEMLCSTLKKQMKYLEQQQDETKQAQEEARRLRSKMKTMEQIELLLQSQRPEVEEMIRDMGVGQSAVEQLAVYCVSL Predict reactive species
Full Name	TRAF interacting protein
Calculated Molecular Weight	53 kDa
Observed Molecular Weight	53 kDa
GenBank Accession Number	BC000310
Gene Symbol	TRAIP
Gene ID (NCBI)	10293
RRID	AB_10638481
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9BWF2
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for TRAIP antibody 10332-1-AP	Download protocol
IHC protocol for TRAIP antibody 10332-1-AP	Download protocol
IF protocol for TRAIP antibody 10332-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Int J Biol Sci ESRG is critical to maintain the cell survival and self-renewal/pluripotency of hPSCs by collaborating with MCM2 to suppress p53 pathway Authors - Shasha Li View Article
mouse	WB	Sci Rep Phycocyanin attenuates pulmonary fibrosis via the TLR2-MyD88-NF-κB signaling pathway. Authors - Chengcheng Li View Article
human	WB,IHC	Biomed Pharmacother TRAIP promotes malignant behaviors and correlates with poor prognosis in liver cancer. Authors - Zhenya Guo KD Validated View Article
human,mouse	WB,IHC	Int Urol Nephrol TRAIP suppressed apoptosis and cell cycle to promote prostate cancer proliferation via TRAF2-PI3K-AKT pathway activation Authors - Yuan Gu KD Validated View Article
human	WB,IHC,IP	Cell Death Dis TRAIP modulates the IGFBP3/AKT pathway to enhance the invasion and proliferation of osteosarcoma by promoting KANK1 degradation. Authors - Mi Li KD Validated View Article
human	IHC,WB	Cancer Gene Ther Silencing TRAIP suppresses cell proliferation and migration/invasion of triple negative breast cancer via RB-E2F signaling and EMT Authors - Yan Zheng KD Validated View Article