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  • Western Blot (WB) analysis of mouse thymus tissue using TRAT1 Polyclonal antibody (13279-1-AP)

    WB analysis of mouse thymus using 13279-1-AP

    mouse thymus tissue were subjected to SDS PAGE followed by western blot with 13279-1-AP (TRAT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using TRAT1 Polyclonal antibody (13279-1-AP)

    WB analysis of Jurkat using 13279-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 13279-1-AP (TRAT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of Jurkat cells using TRAT1 Polyclonal antibody (13279-1-AP)

    IP experiment of Jurkat using 13279-1-AP

    IP result of anti-TRAT1 (IP:13279-1-AP, 4ug; Detection:13279-1-AP 1:300) with Jurkat cells lysate 3000 ug.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using TRAT1 Polyclonal antibody (13279-1-AP)

    IHC staining of human tonsillitis using 13279-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 13279-1-AP (TRAT1 Antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using TRAT1 Polyclonal antibody (13279-1-AP)

    IHC staining of human tonsillitis using 13279-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 13279-1-AP (TRAT1 Antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

TRAT1 Polyclonal antibody

TRAT1 Polyclonal Antibody for WB, IP, IHC, ELISA

Cat No. 13279-1-AP
Publications(1)

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Synonyms

HSPC062, pp29/30, TCRIM, TRAT1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive WB detected inmouse thymus tissue, Jurkat cells
Positive IP detected inJurkat cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 1 publications below

Product Information

13279-1-AP targets TRAT1 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TRAT1 fusion protein Ag4023 Predict reactive species
Full Name T cell receptor associated transmembrane adaptor 1
Calculated Molecular Weight 186 aa, 21 kDa
Observed Molecular Weight 29 kDa
GenBank Accession NumberBC025713
Gene Symbol TRAT1
Gene ID (NCBI) 50852
RRIDAB_10643524
Conjugate Unconjugated
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6PIZ9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for TRAT1 antibody 13279-1-APDownload protocol
IHC protocol for TRAT1 antibody 13279-1-APDownload protocol
IP protocol for TRAT1 antibody 13279-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Sci Rep

Single cell transcriptome sequencing indicates the cellular heterogeneity of small intestine tissue in celiac disease

Authors - Tian Shi
View Article