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TREML4 Polyclonal antibody

TREML4 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 18056-1-AP

Synonyms

TLT 4, TLT4, Trem like transcript 4 protein, TREML4

Validation Data Gallery

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  • Western Blot (WB) analysis of HL-60 cells using TREML4 Polyclonal antibody (18056-1-AP)

    WB analysis of HL-60 using 18056-1-AP

    HL-60 cells were subjected to SDS PAGE followed by western blot with 18056-1-AP (TREML4 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of U-937 cells using TREML4 Polyclonal antibody (18056-1-AP)

    WB analysis of U-937 using 18056-1-AP

    U-937 cells were subjected to SDS PAGE followed by western blot with 18056-1-AP (TREML4 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse thymus tissue using TREML4 Polyclonal antibody (18056-1-AP)

    WB analysis of mouse thymus using 18056-1-AP

    mouse thymus tissue were subjected to SDS PAGE followed by western blot with 18056-1-AP (TREML4 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human spleen tissue using TREML4 Polyclonal antibody (18056-1-AP)

    IHC staining of human spleen using 18056-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 18056-1-AP (TREML4 antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human spleen tissue using TREML4 Polyclonal antibody (18056-1-AP)

    IHC staining of human spleen using 18056-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 18056-1-AP (TREML4 antibody) at dilution of 1:200 (under 40x lens).

Tested Applications

Positive WB detected inHL-60 cells, mouse thymus tissue, U-937 cells
Positive IHC detected inhuman spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 18056-1-AP is TREML4 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TREML4 fusion protein Ag12574
Full Name triggering receptor expressed on myeloid cells-like 4
Calculated Molecular Weight 200 aa, 22 kDa
Observed Molecular Weight 50-55 kDa
GenBank Accession NumberBC101780
Gene Symbol TREML4
Gene ID (NCBI) 285852
RRIDAB_2878488
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for TREML4 antibody 18056-1-APDownload protocol
IHC protocol for TREML4 antibody 18056-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols