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- KD/KO Validated
TRIM29 Polyclonal antibody
TRIM29 Polyclonal Antibody for IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (1)
Applications
WB, IP, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 17542-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, A431 cells, BxPC-3 cells |
| Positive IHC detected in | human skin cancer tissue, human skin tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 7 publications below |
| IHC | See 3 publications below |
| IF | See 2 publications below |
| IP | See 1 publications below |
Product Information
17542-1-AP targets TRIM29 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | TRIM29 fusion protein Ag11600 |
| Full Name | tripartite motif-containing 29 |
| Calculated Molecular Weight | 588 aa, 66 kDa |
| Observed Molecular Weight | 65 kDa |
| GenBank Accession Number | BC017352 |
| Gene Symbol | TRIM29 |
| Gene ID (NCBI) | 23650 |
| RRID | AB_2272412 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TRIM29(Tripartite motif-containing protein 29) is also named as ATDC( Ataxia telangiectasia group D-associated protein).ATDC protein physically interacted with the intermediate filament protein vimentin, a protein kinase C substrate and colocalizing protein, and with an inhibitor of protein kinase C, PKCI1(PMID:7644499). ATDC gene is highly expressed in samples of primary pancreatic cancer and PanIN lesions compared to normal pancreas and chronic pancreatitis and the highly expressed protein in human pancreatic cancer,enhances cancer cell proliferation and in vivo tumorigenicity by upregulation of the Wnt/b-Catenin signaling pathway(Wang, L. etc.2007).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TRIM29 antibody 17542-1-AP | Download protocol |
| IHC protocol for TRIM29 antibody 17542-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Cancer CircIL4R activates the PI3K/AKT signaling pathway via the miR-761/TRIM29/PHLPP1 axis and promotes proliferation and metastasis in colorectal cancer.
| ||
Oncogenesis Galectin-9 promotes a suppressive microenvironment in human cancer by enhancing STING degradation. | ||
PLoS One Ataxia-Telangiectasia Group D Complementing Gene (ATDC) Promotes Lung Cancer Cell Proliferation by Activating NF-κB Pathway. | ||
Cancer Sci Role of deltaNp63(pos) CD44v(pos) cells in the development of N-nitroso-tris-chloroethylurea-induced peripheral-type mouse lung squamous cell carcinomas. | ||
Cancer Biol Ther iTRAQ-based quantitative proteomic analysis of differentially expressed proteins in chemoresistant nasopharyngeal carcinoma. | ||
Cancer Res Treat TRIM29 Overexpression Promotes Proliferation and Survival of Bladder Cancer Cells Through NF-κB Signaling.
|
