Tested Applications
| Positive WB detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 2 publications below |
| IHC | See 1 publications below |
| IF | See 1 publications below |
| IP | See 1 publications below |
Product Information
24369-1-AP targets TRIM67 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag21632 Product name: Recombinant human TRIM67 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 26-102 aa of BC157055 Sequence: VCLPCARTIAVQTPDGEQHLPQPLLLSRGSGLQAGAAAAASLEHDAAAGPACGGAGGSAAGGLGGGAGGGGDHADKL Predict reactive species |
| Full Name | tripartite motif-containing 67 |
| Calculated Molecular Weight | 783 aa, 84 kDa |
| Observed Molecular Weight | 70 kDa |
| GenBank Accession Number | BC157055 |
| Gene Symbol | TRIM67 |
| Gene ID (NCBI) | 440730 |
| RRID | AB_2879510 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q6ZTA4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TRIM67(Tripartite motif-containing protein 67) is also named as TNL and belongs to the belongs to the TRIM/RBCC family. The deduced protein contains an N-terminal RING finger domain, 2 B-boxes, a coiled-coil region, a COS box, an FN3 domain, and a B30.2 domain. TRIM67 shares 65% amino acid identity with TRIM9. This protein has 2 isoforms produced by alternative splicing.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TRIM67 antibody 24369-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Agric Food Chem Dandouchi Polypeptide Alleviates Depressive-like Behavior and Promotes Hippocampal Neurogenesis by Activating the TRIM67/NF-κB Pathway in CUMS-Induced Mice
| ||
Ann Neurol Detection of High-Risk Paraneoplastic Antibodies Against TRIM9 and TRIM67 proteins | ||
Cancer Biol Ther PCK2 promotes invasion and epithelial-to-mesenchymal transition in triple-negative breast cancer by promoting TGF-β/SMAD3 signaling through inhibiting TRIM67-mediated SMAD3 ubiquitination | ||
Int J Mol Sci Ameliorating Effects of TRIM67 against Intestinal Inflammation and Barrier Dysfunction Induced by High Fat Diet in Obese Mice |
