TRIM9 Antibody
Rabbit Polyclonal
| Tested applications | ELISA, WB, IHC |
| Cited applications | |
| Species specificity | human, mouse, rat; other species not tested. |
| Cited species | |
| Positive WB detected in | mouse brain tissue, HepG2 cells |
| Positive IHC detected in | human prostate cancer |
| Recommended dilution | WB: 1:500-1:5000 IHC: 1:50-1:200 |
IP = Immunoprecipitation
mouse brain tissue were subjected to SDS PAGE followed by western blot with 10786-1-AP(TRIM9 Antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human prostate cancer using 10786-1-AP(TRIM9 Antibody) at Dilution 1:100 (under 25x lens)
| Source | Rabbit | Purification method | Antigen affinity purification |
| Isotype | IgG | Storage | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
| Immunogen | TRIM9 fusion protein ag1238 | Full name | tripartite motif-containing 9 |
|
Calculated molecular weight |
80kd |
Observed molecular
weight |
90kd, 61kd |
|
GenBank accession number |
BC013414 | Gene ID (NCBI) | 114088 |
| Gene symbol | TRIM9 | Synonyms | KIAA0282; RNF91; SPRING |
TRIM9(E3 ubiquitin-protein ligase TRIM9) is also named as RNF91 and belongs to the TRIM/RBCC family. TRIM9 protein is a brain-specific E3 ubiquitin ligase. Importantly, TRIM9 is present in LBs found in DLB and PD, suggesting that TRIM9 not only plays roles in the regulation of neuronal functions, but also participates in the formation or breakdown of abnormal inclusions through its ligase activity and besides the striatum and hippocampus, the highest expression of TRIM9 protein is seen in the frontal, temporal and occipital cortices through the western blot (PMID:20085810). It has been reported that TRIM9 is highly expressed in the cerebral cortex in mouse and human brains and that TRIM9 is decreased in damaged brains in patients who have Parkinson disease and dementia with Lewy bodies(PMID:22337885). It has 3 isoforms produced by alternative splicing. This antibody is specific to TRIM9. |
