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TRIOBP Polyclonal antibody

TRIOBP Polyclonal Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, canine and More (1)

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 16124-1-AP

Synonyms

DFNB28, HRIHFB2122, KIAA1662, Protein Tara, TARA, TRIOBP

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using TRIOBP Polyclonal antibody (16124-1-AP)

    WB analysis using 16124-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16124-1-AP (TRIOBP antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of MDCK cells using TRIOBP Polyclonal antibody (16124-1-AP)

    IF Staining of MDCK using 16124-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed MDCK cells using TARA antibody (16124-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, MCF-7 cells
Positive IF detected inMDCK cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16124-1-AP targets TRIOBP in WB, IF, ELISA applications and shows reactivity with human, canine samples.

Tested Reactivity human, canine
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TRIOBP fusion protein Ag9092
Full Name TRIO and F-actin binding protein
Calculated Molecular Weight 2356 aa, 261 kDa
Observed Molecular Weight 68-75 kDa
GenBank Accession NumberBC013278
Gene Symbol TRIOBP
Gene ID (NCBI) 11078
RRIDAB_2209237
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for TRIOBP antibody 16124-1-APDownload protocol
IF protocol for TRIOBP antibody 16124-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Proc Natl Acad Sci U S A

Unbalanced bidirectional radial stiffness gradients within the organ of Corti promoted by TRIOBP.

Authors - Hesam Babahosseini
View Article
humanIF

J Cell Biol

ANKRD24 organizes TRIOBP to reinforce stereocilia insertion points.

Authors - Jocelyn F Krey
View Article
mouseIF

Mol Neurobiol

Essential Role of Sptan1 in Cochlear Hair Cell Morphology and Function Via Focal Adhesion Signaling.

Authors - Qingxiu Yao
View Article
mouseIF

J Cell Sci

GIPC3 couples to MYO6 and PDZ domain proteins and shapes the hair cell apical region

Authors - Paroma Chatterjee
View Article
mouseIF

Elife

Differential regulation of hair cell actin cytoskeleton mediated by SRF and MRTFB

Authors - Ling-Yun Zhou
View Article
human,mouseWB

Cell Mol Life Sci

TRIOBP modulates β-catenin signaling by regulation of miR-29b in idiopathic pulmonary fibrosis

Authors - Lan Wang
View Article