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TRUB1 Polyclonal antibody

TRUB1 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12520-1-AP

Synonyms

PUS4, TRUB1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TRUB1 Polyclonal antibody (12520-1-AP)

    WB analysis using 12520-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12520-1-AP (TRUB1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse liver tissue using TRUB1 Polyclonal antibody (12520-1-AP)

    IP experiment of mouse liver using 12520-1-AP

    IP result of anti-TRUB1 (IP:12520-1-AP, 4ug; Detection:12520-1-AP 1:300) with mouse liver tissue lysate 6400ug.

  • Immunohistochemistry (IHC) staining of human kidney tissue using TRUB1 Polyclonal antibody (12520-1-AP)

    IHC staining of human kidney using 12520-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12520-1-AP (TRUB1 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using TRUB1 Polyclonal antibody (12520-1-AP)

    IHC staining of human kidney using 12520-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12520-1-AP (TRUB1 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using TRUB1 Polyclonal antibody (12520-1-AP)

    IHC staining of mouse testis using 12520-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12520-1-AP (TRUB1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using TRUB1 Polyclonal antibody (12520-1-AP)

    IHC staining of mouse testis using 12520-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12520-1-AP (TRUB1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHEK-293T cells, HepG2 cells
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman kidney tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12520-1-AP targets TRUB1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TRUB1 fusion protein Ag3213
Full Name TruB pseudouridine (psi) synthase homolog 1 (E. coli)
Calculated Molecular Weight 349 aa, 37 kDa
Observed Molecular Weight 37 kDa
GenBank Accession NumberBC030601
Gene Symbol TRUB1
Gene ID (NCBI) 142940
RRIDAB_10640437
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TRUB1 gene encodes a 349-amino acid product, with a VFAVHKPKGPTSA box in positions 71-83 corresponding to motif I of the TruB family (probably involved in conserving protein structure). This protein may be responsible for synthesis of pseudouridine from uracil in transfer RNAs.

Protocols

Product Specific Protocols
WB protocol for TRUB1 antibody 12520-1-APDownload protocol
IHC protocol for TRUB1 antibody 12520-1-APDownload protocol
IP protocol for TRUB1 antibody 12520-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols