Tested Applications
| Positive WB detected in | mouse colon tissue, human brain tissue, mouse brain tissue |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 3 publications below |
Product Information
13103-2-AP targets TSEN2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3742 Product name: Recombinant human TSEN2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 159-465 aa of BC019582 Sequence: SNMEGTAGGERPSVVNGDSGKSGGVGDPREPLGCLQEGSGCHPTTESFEKSVREDASPLPHVCCCKQDALILQRGLHHEDGSQHIGLLHPGDRGPDHEYVLVEEAECAMSEREAAPNEELVQRNRLICRRNPYRIFEYLQLSLEEAFFLVYALGCLSIYYEKEPLTIVKLWKAFTVVQPTFRTTYMAYHYFRSKGWVPKVGLKYGTDLLLYRKGPPFYHASYSVIIELVDDHFEGSLRRPLSWKSLAALSRVSVNVSKELMLCYLIKPSTMTDKEMESPECMKRIKVQEVILSRWVSSRERSDQDDL Predict reactive species |
| Full Name | tRNA splicing endonuclease 2 homolog (S. cerevisiae) |
| Calculated Molecular Weight | 465 aa, 51 kDa |
| Observed Molecular Weight | 51 kDa |
| GenBank Accession Number | BC019582 |
| Gene Symbol | TSEN2 |
| Gene ID (NCBI) | 80746 |
| RRID | AB_2272213 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8NCE0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TSEN2, also named as SEN2, belongs to the tRNA-intron endonuclease family. TSEN2 is a metal ion independent nuclease that cleave the 5' splice site (PMID: 32476018). TSEN2 has 5 isoforms with the molecular mass of 45-53 kDa. TSEN2 is involved in the formation of a human endonuclease complex whose function is the removal of introns from pre-tRNA (PMID: 16595010).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for TSEN2 antibody 13103-2-AP | Download protocol |
| WB protocol for TSEN2 antibody 13103-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell CLP1 Founder Mutation Links tRNA Splicing and Maturation to Cerebellar Development and Neurodegeneration. | ||
Am J Hum Genet Autosomal-Recessive Mutations in the tRNA Splicing Endonuclease Subunit TSEN15 Cause Pontocerebellar Hypoplasia and Progressive Microcephaly. | ||
Genetics An unknown essential function of tRNA splicing endonuclease is linked to the integrated stress response and intron debranching
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