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TTC31 Polyclonal antibody

TTC31 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 21693-1-AP

Synonyms

TPR repeat protein 31, TTC31

Validation Data Gallery

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  • Western Blot (WB) analysis of PC-3 cells using TTC31 Polyclonal antibody (21693-1-AP)

    WB analysis of PC-3 using 21693-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 21693-1-AP (TTC31 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using TTC31 Polyclonal antibody (21693-1-AP)

    WB analysis of PC-3 using 21693-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 21693-1-AP (TTC31 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using TTC31 Polyclonal antibody (21693-1-AP)

    WB analysis of PC-3 using 21693-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 21693-1-AP (TTC31 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human testis tissue using TTC31 Polyclonal antibody (21693-1-AP)

    IHC staining of human testis using 21693-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 21693-1-AP (TTC31 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using TTC31 Polyclonal antibody (21693-1-AP)

    IHC staining of human testis using 21693-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 21693-1-AP (TTC31 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using TTC31 Polyclonal antibody (21693-1-AP)

    IHC staining of human liver using 21693-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 21693-1-AP (TTC31 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using TTC31 Polyclonal antibody (21693-1-AP)

    IHC staining of human liver using 21693-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 21693-1-AP (TTC31 antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of PC-3 cells using TTC31 Polyclonal antibody (21693-1-AP)

    IF Staining of PC-3 using 21693-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed PC-3 cells using 21693-1-AP (TTC31 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inPC-3 cells
Positive IHC detected inhuman testis tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inPC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 21693-1-AP is TTC31 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TTC31 fusion protein Ag16281
Full Name tetratricopeptide repeat domain 31
Calculated Molecular Weight 358 aa, 40 kDa
Observed Molecular Weight 57 kDa
GenBank Accession NumberBC098365
Gene Symbol TTC31
Gene ID (NCBI) 64427
RRIDAB_11182824
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for TTC31 antibody 21693-1-APDownload protocol
IHC protocol for TTC31 antibody 21693-1-APDownload protocol
IF protocol for TTC31 antibody 21693-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols