Filter: AllWBIHCIF/ICC

WB analysis of PC-3 using 21693-1-AP
PC-3 cells were subjected to SDS PAGE followed by western blot with 21693-1-AP (TTC31 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of PC-3 using 21693-1-AP
PC-3 cells were subjected to SDS PAGE followed by western blot with 21693-1-AP (TTC31 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of PC-3 using 21693-1-AP
PC-3 cells were subjected to SDS PAGE followed by western blot with 21693-1-AP (TTC31 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
IHC staining of human testis using 21693-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 21693-1-AP (TTC31 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human testis using 21693-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 21693-1-AP (TTC31 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human liver using 21693-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 21693-1-AP (TTC31 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human liver using 21693-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 21693-1-AP (TTC31 antibody) at dilution of 1:50 (under 40x lens).
IF Staining of PC-3 using 21693-1-AP
Immunofluorescent analysis of (-20℃ Ethanol) fixed PC-3 cells using 21693-1-AP (TTC31 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

TTC31 Polyclonal antibody

Name: TTC31 Polyclonal antibody
Brand: Proteintech
SKU: 21693-1-AP

TTC31 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 21693-1-AP

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

TPR repeat protein 31

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

Proteintech Guarantee

Overview

Tested Applications
Product Information
Protocols

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	PC-3 cells
Positive IHC detected in	human testis tissue, human liver tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	PC-3 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21693-1-AP targets TTC31 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag16281 Product name: Recombinant human TTC31 protein Source: e coli.-derived, PKG Tag: GST Domain: 1-358 aa of BC098365 Sequence: MAPIPKTVGRIKLDCSLRPSCPLEVAAAPKLCKEFGPEDYGEEDIVDFLRRLVESDPQGLHRIHVDGSSGRLQLWHHDYLLGHLDDEGKSTGQSDRGKGAEGLGTYCGLRKSFLYPPQESEPCPQSPSASATFPSVSDSLLQVAMPQKLLVTEEEANRLAEELVAEEERMKQKAEKKRLKKKRQKERKRQERLEQYCGEPKASTTSDGDESPPSSPGNPVQGQCGEEEDSLDLSSTFVSLALRKVGDWPLSARREKGLNQEPQGRGLALQKMGQEEESPPREERPQQSPKEKDLGRLRPQDLLDFAPYPQASPGLLAAALQQSQELAKLGTSFAQNGFYHEAVVLFTQALKLNPQDHR Predict reactive species
Full Name	tetratricopeptide repeat domain 31
Calculated Molecular Weight	358 aa, 40 kDa
Observed Molecular Weight	57 kDa
GenBank Accession Number	BC098365
Gene Symbol	TTC31
Gene ID (NCBI)	64427
RRID	AB_11182824
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q49AM3
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IF protocol for TTC31 antibody 21693-1-AP	Download protocol
IHC protocol for TTC31 antibody 21693-1-AP	Download protocol
WB protocol for TTC31 antibody 21693-1-AP	Download protocol