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TTC32 Polyclonal antibody

TTC32 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25422-1-AP

Synonyms

TPR repeat protein 32, TTC32

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse testis tissue using TTC32 Polyclonal antibody (25422-1-AP)

    WB analysis of mouse testis using 25422-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 25422-1-AP (TTC32 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse testis tissue using TTC32 Polyclonal antibody (25422-1-AP)

    IP experiment of mouse testis using 25422-1-AP

    IP result of anti-TTC32 (IP:25422-1-AP, 4ug; Detection:25422-1-AP 1:300) with mouse testis tissue lysate 4000ug.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using TTC32 Polyclonal antibody (25422-1-AP)

    IHC staining of mouse testis using 25422-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 25422-1-AP (TTC32 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using TTC32 Polyclonal antibody (25422-1-AP)

    IHC staining of mouse testis using 25422-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 25422-1-AP (TTC32 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inmouse testis tissue
Positive IP detected inmouse testis tissue
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 25422-1-AP is TTC32 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TTC32 fusion protein Ag22115
Full Name tetratricopeptide repeat domain 32
Calculated Molecular Weight 151 aa, 17 kDa
Observed Molecular Weight 17-30 kDa
GenBank Accession NumberBC057850
Gene Symbol TTC32
Gene ID (NCBI) 130502
RRIDAB_2880073
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for TTC32 antibody 25422-1-APDownload protocol
IHC protocol for TTC32 antibody 25422-1-APDownload protocol
IP protocol for TTC32 antibody 25422-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols