Filter: AllWBIPIHCIF/ICC

WB analysis using 24594-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 24594-1-AP (TTC37 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 24594-1-AP
MCF-7 cells were subjected to SDS PAGE followed by western blot with 24594-1-AP (TTC37 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 24594-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 24594-1-AP (TTC37 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IP experiment of mouse testis using 24594-1-AP
IP result of anti-TTC37 (IP:24594-1-AP, 4ug; Detection:24594-1-AP 1:300) with mouse testis tissue lysate 4000ug.
IHC staining of human kidney using 24594-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24594-1-AP (TTC37 Antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human tonsillitis using 24594-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 24594-1-AP (TTC37 Antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human kidney using 24594-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24594-1-AP (TTC37 Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human tonsillitis using 24594-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 24594-1-AP (TTC37 Antibody) at dilution of 1:50 (under 10x lens).
IF Staining of A549 using 24594-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using TTC37 antibody (24594-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

TTC37 Polyclonal antibody

Name: TTC37 Polyclonal antibody
Brand: Proteintech
SKU: 24594-1-AP

TTC37 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 24594-1-AP

Publications(4)

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

TPR repeat protein 37, Thespin, Superkiller complex protein 3, SKIC3, Ski3

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

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Overview

Tested Applications
Product Information
Publications (4)
Protocols

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	HEK-293 cells, MCF-7 cells, HeLa cells, HepG2 cells, mouse testis tissue
Positive IP detected in	mouse testis tissue
Positive IHC detected in	human kidney tissue, human tonsillitis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	A549 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 4 publications below

Product Information

24594-1-AP targets TTC37 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag18442 Product name: Recombinant human TTC37 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1215-1564 aa of BC056893 Sequence: VVAGNVAHILDSNHGKKALLYTAVNQLAMGSSSAEDEKNTALKTIQKAALLSPGDPAIWAGLMAACHADDKLALVNNTQPKRIDLYLALLSAVSASIKDEKFFENYNQSLEKWSLSQAVTGLIDTGRISEAETLCTKNLKSNPDQPAVILLLRQVQCKPLLESQKPLPDAVLEELQKTVMSNSTSVPAWQWLAHVYQSQGMMRAAEMCYRKSLQLASQRGSWSGKLSSLLRLALLALKVCMANISNDHWPSLVQEATTEALKLCFCPLAVLLQALLQFKRKMGARETRRLLERVVYQPGYPKSIASTARWYLLRHLYAKDDYELIDVLVNNAKTHGDTRALELNQRLSSQ Predict reactive species
Full Name	tetratricopeptide repeat domain 37
Calculated Molecular Weight	1564 aa, 175 kDa
Observed Molecular Weight	140-150 kDa
GenBank Accession Number	BC056893
Gene Symbol	TTC37
Gene ID (NCBI)	9652
RRID	AB_2879628
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q6PGP7
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TTC37 (tetratricopeptide repeat protein 37), also named as Thespin, encodes 1,564 amino acid protein that contains twenty tetratricopeptide (TPR) repeats. TPR repeat-containing motifs are found in a variety of proteins and may mediate protein-protein interactions and chaperone activity. Although not expressed in liver, TTC37 is a widely expressed protein with highest levels of expression in vascular tissues, lymph node, pituitary, lung and intestine. TTC37 is a component of the SKI complex which is thought to be involved in exosome-mediated RNA decay and associates with transcriptionally active genes in a manner dependent on PAF1 complex (PAF1C). Defects in TTC37 are associated with Trico-Hepato-Enteric (THE) syndrome.

Protocols

Product Specific Protocols
IF protocol for TTC37 antibody 24594-1-AP	Download protocol
IHC protocol for TTC37 antibody 24594-1-AP	Download protocol
IP protocol for TTC37 antibody 24594-1-AP	Download protocol
WB protocol for TTC37 antibody 24594-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human,mouse	WB	J Clin Invest Cytoplasmic RNA quality control failure engages mTORC1-mediated autoinflammatory disease. Authors - Kun Yang View Article
human	WB	PLoS Genet A Comprehensive Genomic Analysis Reveals the Genetic Landscape of Mitochondrial Respiratory Chain Complex Deficiencies. Authors - Masakazu Kohda View Article
human	WB	Mol Cancer Ther Targeting the synthetic lethal relationship between FOCAD and TUT7 represents a potential therapeutic opportunity for TUT4/7 small molecule inhibitors in cancer Authors - Robinson Triboulet View Article
human	WB	EMBO J RNA helicase SKIV2L limits antiviral defense and autoinflammation elicited by the OAS-RNase L pathway Authors - Kun Yang KO Validated View Article