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- KD/KO Validated
TUB Polyclonal antibody
TUB Polyclonal Antibody for IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, IF, ChIP, ELISA
Conjugate
Unconjugated
Cat no : 17928-1-AP
Synonyms
Validation Data Gallery
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Tested Applications
| Positive WB detected in | mouse brain tissue, mouse uterus tissue, Neuro-2a cells, rat brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 2 publications below |
| IF | See 1 publications below |
| ChIP | See 1 publications below |
Product Information
17928-1-AP targets TUB in WB, IP, IHC, IF, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | TUB fusion protein Ag12354 |
| Full Name | tubby homolog (mouse) |
| Calculated Molecular Weight | 561 aa, 62 kDa |
| Observed Molecular Weight | 56 kDa, 62-67 kDa |
| GenBank Accession Number | BC075032 |
| Gene Symbol | TUB |
| Gene ID (NCBI) | 7275 |
| RRID | AB_2212236 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TUB antibody 17928-1-AP | Download protocol |
| IHC protocol for TUB antibody 17928-1-AP | Download protocol |
| IP protocol for TUB antibody 17928-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Cells Differential Roles of Tubby Family Proteins in Ciliary Formation and Trafficking. | ||
Cell Rep NAD+ supplement potentiates tumor-killing function by rescuing defective TUB-mediated NAMPT transcription in tumor-infiltrated T cells.
| ||
Front Neurosci Distribution of ciliary adaptor proteins tubby and TULP3 in the organ of Corti |
