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TXNDC16 Polyclonal antibody

TXNDC16 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF

Conjugate

Unconjugated

Cat no : 21502-1-AP

Synonyms

KIAA1344, TXNDC16

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TXNDC16 Polyclonal antibody (21502-1-AP)

    WB analysis using 21502-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21502-1-AP (TXNDC16 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SMMC-7721 cells using TXNDC16 Polyclonal antibody (21502-1-AP)

    WB analysis of SMMC-7721 using 21502-1-AP

    SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 21502-1-AP (TXNDC16 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using TXNDC16 Polyclonal antibody (21502-1-AP)

    IHC staining of human kidney using 21502-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 21502-1-AP (TXNDC16 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using TXNDC16 Polyclonal antibody (21502-1-AP)

    IHC staining of human kidney using 21502-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 21502-1-AP (TXNDC16 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TXNDC16 Polyclonal antibody (21502-1-AP)

    IF Staining of HeLa using 21502-1-AP

    Immunofluorescent analysis of HeLa cells using 21502-1-AP (TXNDC16 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHEK-293 cells, SMMC-7721 cells, HeLa cells, HepG2 cells, COLO 320 cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 21502-1-AP is TXNDC16 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TXNDC16 fusion protein Ag15946
Full Name thioredoxin domain containing 16
Calculated Molecular Weight 825 aa, 94 kDa
Observed Molecular Weight 100-105 kDa
GenBank Accession NumberBC142650
Gene Symbol TXNDC16
Gene ID (NCBI) 57544
RRIDAB_2878870
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for TXNDC16 antibody 21502-1-APDownload protocol
IHC protocol for TXNDC16 antibody 21502-1-APDownload protocol
IF protocol for TXNDC16 antibody 21502-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols