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KD/KO Validated

UBA2 Polyclonal antibody

UBA2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 15347-1-AP

Publications(2)

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

Anthracycline-associated resistance ARX, ARX, EC:2.3.2.-, HRIHFB2115, SAE2

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

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Filter: AllWBIPIHCIF/ICC

WB analysis using 15347-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 15347-1-AP (UBA2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of human brain using 15347-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 15347-1-AP (UBA2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of Jurkat using 15347-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 15347-1-AP (UBA2 antibody) at dilution of 1:700 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of human brain using 15347-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 15347-1-AP (UBA2 antibody) at dilution of 1:700 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IP experiment of Jurkat using 15347-1-AP
IP result of anti-UBA2 (IP:15347-1-AP, 4ug; Detection:15347-1-AP 1:10000) with Jurkat cells lysate 1360 ug.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human breast cancer using 15347-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15347-1-AP (UBA2 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of HeLa using 15347-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using UBA2 antibody (15347-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Overview

Tested Applications
Product Information
Publications (2)
Protocols

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	A549 cells, human brain tissue, Jurkat cells, HeLa cells, K-562 cells
Positive IP detected in	Jurkat cells
Positive IHC detected in	human breast cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:6000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:300-1:1200
Immunofluorescence (IF)/ICC	IF/ICC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15347-1-AP targets UBA2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag7586 Product name: Recombinant human UBA2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 294-640 aa of BC003153 Sequence: TNASDQQNEPQLGLKDQQVLDVKSYARLFSKSIETLRVHLAEKGDGAELIWDKDDPSAMDFVTSAANLRMHIFSMNMKSRFDIKSMAGNIIPAIATTNAVIAGLIVLEGLKILSGKIDQCRTIFLNKQPNPRKKLLVPCALDPPNPNCYVCASKPEVTVRLNVHKVTVLTLQDKIVKEKFAMVAPDVQIEDGKGTILISSEEGETEANNHKKLSEFGIRNGSRLQADDFLQDYTLLINILHSEDLGKDVEFEVVGDAPEKVGPKQAEDAAKSITNGSDDGAQPSTSTAQEQDDVLIVDSDEEDSSNNADVSEEERSRKRKLDEKENLSAKRSRIEQKEELDDVIALD Predict reactive species
Full Name	ubiquitin-like modifier activating enzyme 2
Calculated Molecular Weight	71 kDa
Observed Molecular Weight	90 kDa
GenBank Accession Number	BC003153
Gene Symbol	UBA2
Gene ID (NCBI)	10054
RRID	AB_2211586
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9UBT2
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IF protocol for UBA2 antibody 15347-1-AP	Download protocol
IHC protocol for UBA2 antibody 15347-1-AP	Download protocol
IP protocol for UBA2 antibody 15347-1-AP	Download protocol
WB protocol for UBA2 antibody 15347-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

Species	Application	Title
human	WB,IHC,IF	Cell Death Differ Inhibition of Drp1 SUMOylation by ALR protects the liver from ischemia-reperfusion injury. Authors - Jing Huang KD Validated View Article
mouse	WB,IHC,IF	Oncoimmunology Mild microwave hyperthermia promotes mitotic catastrophe, induces time-delayed cGAS-STING activation and restores sensitivity to anti-PDL1 therapy in Pan02 pancreatic cancer model. Authors - Na Li View Article