Tested Applications
| Positive WB detected in | HEK-293 cells, human brain tissue |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | mouse testis tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | SH-SY5Y cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
13211-1-AP targets UBA6 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4000 Product name: Recombinant human UBA6 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 87-389 aa of BC031637 Sequence: AVTIHDTEKCQAWDLGTNFFLSEDDVVNKRNRAEAVLKHIAELNPYVHVTSSSVPFNETTDLSFLDKYQCVVLTEMKLPLQKKINDFCRSQCPPIKFISADVHGIWSRLFCDFGDEFEVLDTTGEEPKEIFISNITQANPGIVTCLENHPHKLETGQFLTFREINGMTGLNGSIQQITVISPFSFSIGDTTELEPYLHGGIAVQVKTPKTVFFESLERQLKHPKCLIVDFSNPEAPLEIHTAMLALDQFQEKYSRKPNVGCQQDSEELLKLATSISETLEEKVTIEIYGCPNICLLIHKCSVY Predict reactive species |
| Full Name | ubiquitin-like modifier activating enzyme 6 |
| Calculated Molecular Weight | 1052 aa, 118 kDa |
| Observed Molecular Weight | 118 kDa |
| GenBank Accession Number | BC031637 |
| Gene Symbol | UBA6 |
| Gene ID (NCBI) | 55236 |
| RRID | AB_2211747 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | A0AVT1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
UBA6(Ubiquitin-like modifier-activating enzyme 6) is also named as MOP4, UBE1L2 and belongs to the ubiquitin-activating E1 family. It probably acts as a single molecule like UBE1 and not as a heterodimeric E1 complex, as formed by AOS1/Uba2 and APP-BP1/Uba3. UBA6 is even more restricted in distribution because it is only found in vertebrates but not in lower organisms so that it might have a specialized role in tissues of higher organisms, and in particular, in the testis, where it is expressed by far most abundantly.(PMID:17580310). It has 4 isoforms produced by alternative splicing. This antibody recognizes the full-length(118 kda) and the truncated isoform CRA_b (60-70 kDa) of UBA6 (NCBI).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for UBA6 antibody 13211-1-AP | Download protocol |
| IHC protocol for UBA6 antibody 13211-1-AP | Download protocol |
| IP protocol for UBA6 antibody 13211-1-AP | Download protocol |
| WB protocol for UBA6 antibody 13211-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
| Species | Application | Title |
|---|---|---|
Nat Commun Mammalian tRNA acetylation determines translation efficiency and tRNA quality control. | ||
Cell Rep Lactylation orchestrates ubiquitin-independent degradation of cGAS and promotes tumor growth
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