Filter: AllWBIPIHCIF/ICC

WB analysis of human heart using 12385-1-AP
human heart tissue were subjected to SDS PAGE followed by western blot with 12385-1-AP (UBAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human testis using 12385-1-AP
human testis tissue were subjected to SDS PAGE followed by western blot with 12385-1-AP (UBAP1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of A431 using 12385-1-AP
A431 cells were subjected to SDS PAGE followed by western blot with 12385-1-AP (UBAP1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of mouse thymus using 12385-1-AP
mouse thymus tissue were subjected to SDS PAGE followed by western blot with 12385-1-AP (UBAP1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of human testis using 12385-1-AP
human testis tissue were subjected to SDS PAGE followed by western blot with 12385-1-AP (UBAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human testis using 12385-1-AP
human testis tissue were subjected to SDS PAGE followed by western blot with 12385-1-AP (UBAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse lung using 12385-1-AP
mouse lung tissue were subjected to SDS PAGE followed by western blot with 12385-1-AP (UBAP1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 12385-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 12385-1-AP (UBAP1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of human spleen using 12385-1-AP
human spleen tissue were subjected to SDS PAGE followed by western blot with 12385-1-AP (UBAP1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of mouse testis using 12385-1-AP
mouse testis tissue were subjected to SDS PAGE followed by western blot with 12385-1-AP (UBAP1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of Y79 using 12385-1-AP
Y79 cells were subjected to SDS PAGE followed by western blot with 12385-1-AP (UBAP1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of human lung using 12385-1-AP
human lung tissue were subjected to SDS PAGE followed by western blot with 12385-1-AP (UBAP1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
IP experiment of mouse brain using 12385-1-AP
IP result of anti-UBAP1 (IP:12385-1-AP, 4ug; Detection:12385-1-AP 1:500) with mouse brain tissue lysate 4000ug.
IHC staining of human lung cancer using 12385-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12385-1-AP (UBAP1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 12385-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12385-1-AP (UBAP1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung using 12385-1-AP
Immunohistochemical analysis of paraffin-embedded human lung using 12385-1-AP (UBAP1 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human lung using 12385-1-AP
Immunohistochemical analysis of paraffin-embedded human lung using 12385-1-AP (UBAP1 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human lung cancer using 12385-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer using 12385-1-AP (UBAP1 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human lung cancer using 12385-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer using 12385-1-AP (UBAP1 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human heart using 12385-1-AP
Immunohistochemical analysis of paraffin-embedded human heart using 12385-1-AP (UBAP1 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human heart using 12385-1-AP
Immunohistochemical analysis of paraffin-embedded human heart using 12385-1-AP (UBAP1 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human lung using 12385-1-AP
Immunohistochemical analysis of paraffin-embedded human lung using 12385-1-AP (UBAP1 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human lung using 12385-1-AP
Immunohistochemical analysis of paraffin-embedded human lung using 12385-1-AP (UBAP1 antibody) at dilution of 1:50 (under 40x lens).
IF Staining of A431 using 12385-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using UBAP1 antibody (12385-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Featured Product
KD/KO Validated

UBAP1 Polyclonal antibody

Name: UBAP1 Polyclonal antibody
Brand: Proteintech
SKU: 12385-1-AP

UBAP1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 12385-1-AP

Publications(17)

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

UBAP-1, UBAP 1, UBAP, UAP, Nasopharyngeal carcinoma-associated gene 20 protein

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

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Overview

Tested Applications
Product Information
Publications (17)
Protocols

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	human heart tissue, A431 cells, human lung tissue, human spleen tissue, human testis tissue, mouse brain tissue, mouse lung tissue, mouse testis tissue, mouse thymus tissue, Y79 cells
Positive IP detected in	mouse brain tissue
Positive IHC detected in	human lung cancer tissue, human heart tissue, human lung tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	A431 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2400
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 17 publications below
IF	See 1 publications below
IP	See 2 publications below

Product Information

12385-1-AP targets UBAP1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag3055 Product name: Recombinant human UBAP1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 203-502 aa of BC020950 Sequence: GGSGSVLQDEEVLASLERATLDFKPLHKPNGFITLPQLGNCEKMSLSSKVSLPPIPAVSNIKSLSFPKLDSDDSNQKTAKLASTFHSTSCLRNGTFQNSLKPSTQSSASELNGHHTLGLSALNLDSGTEMPALTSSQMPSLSVLSVCTEESSPPNTGPTVTPPNFSVSQVPNMPSCPQAYSELQMLSPSERQCVETVVNMGYSYECVLRAMKKKGENIEQILDYLFAHGQLCEKGFDPLLVEEALEMHQCSEEKMMEFLQLMSKFKEMGFELKDIKEVLLLHNNDQDNALEDLMARAGAS Predict reactive species
Full Name	ubiquitin associated protein 1
Calculated Molecular Weight	502 aa, 55 kDa
Observed Molecular Weight	55 kDa
GenBank Accession Number	BC020950
Gene Symbol	UBAP1
Gene ID (NCBI)	51271
RRID	AB_2211886
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9NZ09
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

UBAP1, also named as NAG20, is a 55 kDa protein which contains 2 putative tandem UBA domains at the C terminus, a coiled-coil domain, 2 possible N-glycosylation sites, 5 PKC phosphorylation sites, 7 casein kinase II phosphorylation sites, and 3 N-myristoylation sites. UBAP1 might be a potential effective diagnosis candidate for NPC and decreased expression of UBAP1 protein is a possible point of dysfunction along the pathogenesis pathway for NPC that may contribute to malignant transformation.

Protocols

Product Specific Protocols
IF protocol for UBAP1 antibody 12385-1-AP	Download protocol
IHC protocol for UBAP1 antibody 12385-1-AP	Download protocol
IP protocol for UBAP1 antibody 12385-1-AP	Download protocol
WB protocol for UBAP1 antibody 12385-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	EMBO J A Pseudomonas aeruginosa TIR effector mediates immune evasion by targeting UBAP1 and TLR adaptors. Authors - Paul Rc Imbert View Article
human	WB	Curr Biol UBAP1 is a component of an endosome-specific ESCRT-I complex that is essential for MVB sorting. Authors - Stefani Flavia F View Article
human	WB	J Cell Biol VPS37A directs ESCRT recruitment for phagophore closure. Authors - Yoshinori Takahashi View Article
mouse	WB,IF	Cell Rep ESCRT-I protein UBAP1 controls ventricular expansion and cortical neurogenesis via modulating adherens junctions of radial glial cells Authors - Danping Lu View Article
human	WB	Cell Rep The Dynamics of TGF-β Signaling Are Dictated by Receptor Trafficking via the ESCRT Machinery. Authors - Daniel S J Miller View Article
human	WB, IP	Cell Rep Interplay of Endosomal pH and Ligand Occupancy in Integrin α5β1 Ubiquitination, Endocytic Sorting, and Cell Migration. Authors - Dmitri Kharitidi View Article