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Tested Applications
| Positive WB detected in | HEK-293 cells, mouse lung tissue |
| Positive IP detected in | mouse kidney tissue |
| Positive IHC detected in | human hepatocirrhosis tissue, human spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 10 publications below |
| IHC | See 1 publications below |
| IF | See 2 publications below |
| CoIP | See 1 publications below |
Product Information
19563-1-AP targets UBASH3B in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | ubiquitin associated and SH3 domain containing, B |
| Calculated Molecular Weight | 73 kDa |
| Observed Molecular Weight | 66-70 kDa |
| GenBank Accession Number | NM_032873 |
| Gene Symbol | UBASH3B |
| Gene ID (NCBI) | 84959 |
| RRID | AB_10643379 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8TF42 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
UBASH3B, also named STS1 or TULA-2, is a protein that contains a ubiquitin-associated domain at the N-terminus, a SH3 domain, and a C-terminal domain with similarities to the catalytic motif of phosphoglycerate mutase. TULA-2 is supposed to form homodimer and interact with ubiquitin and mono-ubiquitinated proteins. TULA-2 functions to promote accumulation of activated target receptors, such as T-cell receptors and EGFR on the cell surface.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for UBASH3B antibody 19563-1-AP | Download protocol |
| IHC protocol for UBASH3B antibody 19563-1-AP | Download protocol |
| IP protocol for UBASH3B antibody 19563-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Oncogenic Mutations Rewire Signaling Pathways by Switching Protein Recruitment to Phosphotyrosine Sites. | ||
Biochimie Ubash3b promotes TPA-mediated suppression of leukemogenesis through accelerated downregulation of PKCδ protein.
| ||
Front Immunol Targeting the Cbl-b-Notch1 axis as a novel immunotherapeutic strategy to boost CD8+ T-cell responses
| ||
Int J Mol Sci STS1 and STS2 Phosphatase Inhibitor Baicalein Enhances the Expansion of Hematopoietic and Progenitor Stem Cells and Alleviates 5-Fluorouracil-Induced Myelosuppression | ||
Cell Rep PSGL-1 attenuates early TCR signaling to suppress CD8+ T cell progenitor differentiation and elicit terminal CD8+ T cell exhaustion |
