UBC9 Antibody
Rabbit Polyclonal
Catalog number: 51018-2-AP
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Applications
| Tested applications | ELISA, WB, IHC, IP |
| Cited applications | |
| Species specificity | human, mouse; other species not tested. |
| Cited species | |
| Positive WB detected in | mouse thymus tissue |
| Positive IHC detected in | human prostate cancer |
| Recommended dilution | WB: 1:1000-1:10000 IHC: 1:50-1:200 |
Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
IP = Immunoprecipitation
Images
IP result of anti-UBE2I (51018-2-AP for IP and Detection) with mouse heart tissue lysate.
mouse thymus tissue were subjected to SDS PAGE followed by western blot with 51018-2-AP(UBC9 Antibody) at dilution of 1:2000
Immunohistochemistry of paraffin-embedded human prostate cancer using 51018-2-AP(UBC9 Antibody) at Dilution 1:100 (under 10x lens)
Product Information
| Source | Rabbit | Purification method | Antigen affinity purification |
| Isotype | IgG | Storage | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
Immunogen Information
| Immunogen | Peptide | Full name | ubiquitin-conjugating enzyme E2I (UBC9 homolog, yeast) |
|
Calculated molecular weight |
18kd |
Observed molecular
weight |
18kd |
|
GenBank accession number |
BC051289 | Gene ID (NCBI) | 7329 |
| Gene symbol | UBE2I | Synonyms | C358B7.1; P18; UBC9 |
Background
UBE2I is also named as UBC9, UBCE9 and belongs to the ubiquitin-conjugating enzyme family. It is a homologue of the E2 ubiquitin conjugating enzyme and participates in the covalent linking of SUMO-1 molecule to the target protein. This protein is present at a high level in spleen and lung. Moderate level of UBE2I is detected in kidney and liver. Low amount of UBE2I is observed in brain, whereas the 18 kDa band of UBE2I is barely visible or absent in heart and skeletal muscle. In heart and muscle extracts the UBE2I antibodies recognizes a 38 kDa protein band,but this band is not visible in extracts of other rat tissues(PMID:14739995). |
