UBE2F Antibody
Rabbit Polyclonal
Catalog number: 17056-1-AP
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Applications
| Tested applications | ELISA, WB |
| Cited applications | |
| Species specificity | human, mouse, rat; other species not tested. |
| Cited species | |
| Positive WB detected in | Jurkat cells, mouse ovary tissue |
| Recommended dilution | WB: 1:500-1:5000 |
Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
IP = Immunoprecipitation
Images
Jurkat cells were subjected to SDS PAGE followed by western blot with 17056-1-AP(UBE2F Antibody) at dilution of 1:1000
Product Information
| Source | Rabbit | Purification method | Antigen affinity purification |
| Isotype | IgG | Storage | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
Immunogen Information
| Immunogen | UBE2F fusion protein ag10764 | Full name | ubiquitin-conjugating enzyme E2F (putative) |
|
Calculated molecular weight |
185aa,21kd |
Observed molecular
weight |
21kd |
|
GenBank accession number |
BC010549 | Gene ID (NCBI) | 140739 |
| Gene symbol | UBE2F | Synonyms | MGC18120; NCE2 |
Background
UBE2F(Ubiquitin-conjugating enzyme E2 F) is also named as NCE2(NEDD8-conjugating enzyme 2) and belongs to the ubiquitin-conjugating enzyme family.The canonical uBE2M catalyses the modification of most cullins, whereas uBE2F is specifically responsible for cuL5 neddylation. uBE2F is regulated differently than uBE2M, supporting the idea that neddylation is used to fine-tune ubiquitin-chain formation by different cullin-dependent E3s(PMID:19465892).This antibody is specific to UBE2F. |
