UBE2I-Specific Antibody
Rabbit Polyclonal
Catalog number: 14837-1-AP
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Applications
| Tested applications | ELISA, WB, IHC |
| Cited applications | |
| Species specificity | human, mouse, rat; other species not tested. |
| Cited species | |
| Positive WB detected in | HEK-293 cells, human testis tissue |
| Positive IHC detected in | human lung cancer |
| Recommended dilution | WB: 1:500-1:5000 IHC: 1:20-1:200 |
Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
IP = Immunoprecipitation
Images
HEK-293 cells were subjected to SDS PAGE followed by western blot with 14837-1-AP(UBE2I-Specific Antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human lung cancer using 14837-1-AP(UBE2I-Specific Antibody) at Dilution 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human lung cancer using 14837-1-AP(UBE2I-Specific Antibody) at Dilution 1:50 (under 40x lens)
Product Information
| Source | Rabbit | Purification method | Antigen affinity purification |
| Isotype | IgG | Storage | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
Immunogen Information
| Immunogen | Peptide | Full name | ubiquitin-conjugating enzyme E2I (UBC9 homolog, yeast) |
|
Calculated molecular weight |
18kd |
Observed molecular
weight |
18kd |
|
GenBank accession number |
NM_003345 | Gene ID (NCBI) | 7329 |
| Gene symbol | UBE2I | Synonyms | C358B7.1; P18; UBC9 |
Background
UBE2I is also named as UBC9, UBCE9 and belongs to the ubiquitin-conjugating enzyme family. It is a homologue of the E2 ubiquitin conjugating enzyme and participates in the covalent linking of SUMO-1 molecule to the target protein. This protein is present at a high level in spleen and lung. Moderate level of UBE2I is detected in kidney and liver. Low amount of UBE2I is observed in brain, whereas the 18 kDa band of UBE2I is barely visible or absent in heart and skeletal muscle. In heart and muscle extracts the UBE2I antibodies recognizes a 38 kDa protein band,but this band is not visible in extracts of other rat tissues(PMID:14739995). This antibody is specific to UBE2I. |
