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UBC9-Specific Polyclonal antibody

UBC9-Specific Polyclonal Antibody for WB, FC, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, FC, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14837-1-AP

Synonyms

C358B7.1, E2,UBE2I, P18, SUMO conjugating enzyme UBC9, SUMO protein ligase, UBC9, UBC9-Specific, UBCE9, UBE2I, Ubiquitin carrier protein 9, Ubiquitin carrier protein I, Ubiquitin protein ligase I

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using UBC9-Specific Polyclonal antibody (14837-1-AP)

    WB analysis using 14837-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14837-1-AP (UBC9-Specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using UBC9-Specific Polyclonal antibody (14837-1-AP)

    IHC staining of human lung cancer using 14837-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14837-1-AP (UBC9-Specific antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using UBC9-Specific Polyclonal antibody (14837-1-AP)

    IHC staining of human lung cancer using 14837-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14837-1-AP (UBC9-Specific antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Flow cytometry (FC) experiment of HepG2 cells using UBC9-Specific Polyclonal antibody (14837-1-AP)

    FC experiment of HepG2 using 14837-1-AP

    1X10^6 HepG2 cells were stained with 0.2ug UBC9-Specific antibody (14837-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

Tested Applications

Positive WB detected inHEK-293 cells, Jurkat cells, U-937 cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14837-1-AP targets UBC9-Specific in WB, IF, FC, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name ubiquitin-conjugating enzyme E2I (UBC9 homolog, yeast)
Calculated Molecular Weight 18 kDa
Observed Molecular Weight 18 kDa
GenBank Accession NumberNM_003345
Gene Symbol UBE2I
Gene ID (NCBI) 7329
RRIDAB_2272479
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

UBC9 is also named as UBE2I, UBCE9 and belongs to the ubiquitin-conjugating enzyme family. It is a homologue of the E2 ubiquitin conjugating enzyme and participates in the covalent linking of SUMO-1 molecule to the target protein. This protein is present at a high level in spleen and lung. Moderate level of UBC9 is detected in kidney and liver. Low amount of UBC9 is observed in brain, whereas the 18 kDa band of UBC9 is barely visible or absent in heart and skeletal muscle. In heart and muscle extracts the UBC9 antibodies recognizes a 38 kDa protein band,but this band is not visible in extracts of other rat tissues(PMID:14739995).

Protocols

Product Specific Protocols
WB protocol for UBC9-Specific antibody 14837-1-APDownload protocol
IHC protocol for UBC9-Specific antibody 14837-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

Nat Commun

Identification of proximal SUMO-dependent interactors using SUMO-ID.

Authors - Orhi Barroso-Gomila
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Praveen (Verified Customer) (03-13-2019)

Excellent

  • Applications: Western Blot,
  • Cell Tissue Type: AC16
FH

Shubham (Verified Customer) (03-12-2019)

Very good antibody with a prominent band.

  • Applications: Western Blot,
  • Cell Tissue Type: AC16
UBC9-Specific Antibody Western Blot, validation ( dilution) in AC16 (Cat no:14837-1-AP)