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  • KD/KO Validated

UBE2L3 Polyclonal antibody

UBE2L3 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14415-1-AP

Synonyms

E2 F1, L UBC, UBCE7, UBCH7, UbcM4, UBE2L3, Ubiquitin carrier protein L3, Ubiquitin protein ligase L3



Tested Applications

Positive WB detected inHeLa cells, A431 cells, HEK-293 cells, K-562 cells, MCF-7 cells, mouse kidney tissue, mouse testis tissue, NIH/3T3 cells, rat kidney tissue
Positive IP detected inmouse kidney tissue
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14415-1-AP targets UBE2L3 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen UBE2L3 fusion protein Ag5758
Full Name ubiquitin-conjugating enzyme E2L 3
Calculated Molecular Weight 18 kDa
Observed Molecular Weight 18 kDa
GenBank Accession NumberBC053368
Gene Symbol UBE2L3
Gene ID (NCBI) 7332
RRIDAB_2210891
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

UBE2L3(Ubiquitin-conjugating enzyme E2 L3) is also named as UBCE7, UBCH7 and belongs to the ubiquitin-conjugating enzyme family. It regulates nuclear hormone receptors transcriptional activity and may play a role in myelopoiesis.UBE2L3 is associated with susceptibility to systemic lupus erythematosus (SLE) and rheumatoid arthritis in European ancestry populations(PMID:22045845).

Protocols

Product Specific Protocols
WB protocol for UBE2L3 antibody 14415-1-APDownload protocol
IHC protocol for UBE2L3 antibody 14415-1-APDownload protocol
IP protocol for UBE2L3 antibody 14415-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IP

Bioengineered

E2F transcription factor 1 (E2F1) promotes the transforming growth factor TGF-β1 induced human cardiac fibroblasts differentiation through promoting the transcription of CCNE2 gene

Authors - Rongheng Liao
  • KD Validated
humanWB

J Cell Sci

A specific subset of E2 ubiquitin-conjugating enzymes regulate Parkin activation and mitophagy differently.

Authors - Fabienne C Fiesel
pigWB

Front Immunol

Foot-and-Mouth Disease Virus Counteracts on Internal Ribosome Entry Site Suppression by G3BP1 and Inhibits G3BP1-Mediated Stress Granule Assembly via Post-Translational Mechanisms.

Authors - Xu Ye
pigWB

Mol Cell Proteomics

The mechano-ubiquitinome of articular cartilage: Differential ubiquitination and activation of a group of ER-associated DUBs and ER stress regulators

Authors - Nitchakarn Kaokhum
mouseWB,IHC

Cell Commun Signal

Metformin alleviates bone loss in ovariectomized mice through inhibition of autophagy of osteoclast precursors mediated by E2F1

Authors - Xudong Xie
humanWB

Mediators Inflamm

UBC Mediated by SEPT6 Inhibited the Progression of Prostate Cancer.

Authors - Ruochen Zhang
  • KD Validated