UBL4B Polyclonal antibody

UBL4B Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 14509-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

Ubiquitin-like protein 4B, Ubiquitin like protein 4B, ubiquitin like 4B

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

SKU:

Freight/Packing: -

Quantity

- +

Filter: AllWBIF/ICC

WB analysis of human testis using 14509-1-AP
human testis tissue were subjected to SDS PAGE followed by western blot with 14509-1-AP (UBL4B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of human testis using 14509-1-AP
human testis tissue were subjected to SDS PAGE followed by western blot with 14509-1-AP (UBL4B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of HepG2 using 14509-1-AP
Immunofluorescent analysis of HepG2 cells, using UBL4B antibody 14509-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of HeLa using 14509-1-AP
Immunofluorescent analysis of Hela cells, using UBL4B antibody 14509-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Positive WB detected in	human testis tissue
Positive IF/ICC detected in	HepG2 cells, Hela cells

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunofluorescence (IF)/ICC	IF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

14509-1-AP targets UBL4B in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag5968 Product name: Recombinant human UBL4B protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-174 aa of BC058929 Sequence: MFLTVKLLLGQRCSLKVSGQESVATLKRLVSRRLKVPEEQQHLLFRGQLLEDDKHLSDYCIGPNASINVIMQPLEKMALKEAHQPQTQPLWHQLGLVLAKHFEPQDAKAVLQLLRQEHEERLQKISLEHLEQLAQYLLAEEPHVEPAGERELEAKARPQSSCDMEEKEEAAADQ Predict reactive species
Full Name	ubiquitin-like 4B
Calculated Molecular Weight	20 kDa
Observed Molecular Weight	22-25 kDa
GenBank Accession Number	BC058929
Gene Symbol	UBL4B
Gene ID (NCBI)	164153
RRID	AB_10640905
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q8N7F7
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Product Specific Protocols
IF protocol for UBL4B antibody 14509-1-AP	Download protocol
WB protocol for UBL4B antibody 14509-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Species	Application	Title
human	WB	Andrology The MAEL expression in mitochondria of human spermatozoa and the association with asthenozoospermia Authors - Yu-Sheng Cheng View Article