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BMSC UbP Polyclonal antibody

BMSC UbP Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 11564-1-AP

Synonyms

BMSC UbP, BMSCUBP, TCBA1, Ubiquitin like protein 7, Ubiquitin like protein SB132, UBL7

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse testis tissue using BMSC UbP Polyclonal antibody (11564-1-AP)

    WB analysis of mouse testis using 11564-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 11564-1-AP (BMSC UbP antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using BMSC UbP Polyclonal antibody (11564-1-AP)

    WB analysis of HeLa using 11564-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 11564-1-AP (BMSC UbP antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using BMSC UbP Polyclonal antibody (11564-1-AP)

    WB analysis of Jurkat using 11564-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 11564-1-AP (BMSC UbP antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using BMSC UbP Polyclonal antibody (11564-1-AP)

    WB analysis of HepG2 using 11564-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 11564-1-AP (BMSC UbP antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat testis tissue using BMSC UbP Polyclonal antibody (11564-1-AP)

    WB analysis of rat testis using 11564-1-AP

    rat testis tissue were subjected to SDS PAGE followed by western blot with 11564-1-AP (BMSC UbP antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using BMSC UbP Polyclonal antibody (11564-1-AP)

    IP experiment of HeLa using 11564-1-AP

    IP result of anti-BMSC UbP (IP:11564-1-AP, 4ug; Detection:11564-1-AP 1:1000) with HeLa cells lysate 2000ug.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using BMSC UbP Polyclonal antibody (11564-1-AP)

    IHC staining of human gliomas using 11564-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas using 11564-1-AP (BMSC UbP antibody) at dilution of 1:50 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using BMSC UbP Polyclonal antibody (11564-1-AP)

    IF Staining of HepG2 using 11564-1-AP

    Immunofluorescent analysis of HepG2 cells using 11564-1-AP (BMSC UbP antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inmouse testis tissue, HeLa cells, HepG2 cells, Jurkat cells, rat testis tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11564-1-AP targets BMSC UbP in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BMSC UbP fusion protein Ag2130
Full Name ubiquitin-like 7 (bone marrow stromal cell-derived)
Calculated Molecular Weight 380 aa, 41 kDa
Observed Molecular Weight 41 kDa
GenBank Accession NumberBC030055
Gene Symbol UBL7
Gene ID (NCBI) 84993
RRIDAB_2212080
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Ubiquitin is an important cellular signal that targets proteins for degradation or regulates their functions. UBL7 (ORF name SB132) encodes ubiquitin-like protein7 also named bone marrow stromal cell ubiquitin-like protein (BMSC-UbP) capable of binding ubiquitin. BMSC-UbP protein is derived from bone marrow stromal cells containing a ubiquitin-associated (UBA) domain at the C terminus that has been implicated in linking cellular processes and the ubiquitin system. BMSC-UbP mRNA is widely expressed in human multiple tissues and various tumor cell lines and is decreased in BMSC stimulated with phorbol myristate acetate (PMA) suggesting a role in regulation of BMSC function.

Protocols

Product Specific Protocols
WB protocol for BMSC UbP antibody 11564-1-APDownload protocol
IHC protocol for BMSC UbP antibody 11564-1-APDownload protocol
IF protocol for BMSC UbP antibody 11564-1-APDownload protocol
IP protocol for BMSC UbP antibody 11564-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols