UHRF1 Antibody
Rabbit Polyclonal
Catalog number: 21402-1-AP
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Applications
| Tested applications | ELISA, WB, IF |
| Cited applications | |
| Species specificity | human; other species not tested. |
| Cited species | |
| Positive WB detected in | HeLa cells, MCF7 cells |
| Positive IF detected in | HepG2 cells |
| Recommended dilution | WB: 1:500-1:5000 IF: 1:20-1:50 |
Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
IP = Immunoprecipitation
Images
HeLa cells were subjected to SDS PAGE followed by western blot with 21402-1-AP(UHRF1 Antibody) at dilution of 1:1000
Immunofluorescent analysis of HepG2 cells, using UHRF1 antibody 21402-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
Product Information
| Source | Rabbit | Purification method | Antigen affinity purification |
| Isotype | IgG | Storage | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
Immunogen Information
| Immunogen | UHRF1 fusion protein ag16111 | Full name | ubiquitin-like with PHD and ring finger domains 1 |
|
Calculated molecular weight |
806aa,91kd |
Observed molecular
weight |
90kd |
|
GenBank accession number |
BC113875 | Gene ID (NCBI) | 29128 |
| Gene symbol | UHRF1 | Synonyms | FLJ21925; ICBP90; MGC138707; Np95; RNF106; hNP95 |
Background
UHRF1, also named as ICBP90, NP95 and RNF106, is a putative E3 ubiquitin-protein ligase. It may participate in methylation-dependent transcriptional regulation. UHRF1 binds to inverted 5'-CCAAT-3' box 2 in the TOP2A promoter, and activates TOP2A expression. It is important for G1/S transition and may be involved in DNA repair and chromosomal stability. UHRF1's ability to repress its direct target gene expression is dependent on PHD(UHRF1) binding to unmodified H3R2. |
