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SUN1 Polyclonal antibody

SUN1 Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 24568-1-AP

Synonyms

KIAA0810, Protein unc 84 homolog A, Sad1/unc 84 protein like 1, SUN1, unc 84 homolog A (C. elegans), UNC84A

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SUN1 Polyclonal antibody (24568-1-AP)

    WB analysis using 24568-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24568-1-AP (SUN1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using SUN1 Polyclonal antibody (24568-1-AP)

    WB analysis of Jurkat using 24568-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 24568-1-AP (SUN1 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using SUN1 Polyclonal antibody (24568-1-AP)

    WB analysis of K-562 using 24568-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 24568-1-AP (SUN1 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of Jurkat cells using SUN1 Polyclonal antibody (24568-1-AP)

    IP experiment of Jurkat using 24568-1-AP

    IP result of anti-SUN1 (IP:24568-1-AP, 4ug; Detection:24568-1-AP 1:1000) with Jurkat cells lysate 3200ug.

Tested Applications

Positive WB detected inA431 cells, Jurkat cells, K-562 cells, HeLa cells
Positive IP detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 24568-1-AP is SUN1 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SUN1 fusion protein Ag21117
Full Name unc-84 homolog A (C. elegans)
Calculated Molecular Weight 90 kDa
Observed Molecular Weight 90-100 kDa
GenBank Accession NumberBC013613
Gene Symbol UNC84A
Gene ID (NCBI) 23353
RRIDAB_2879612
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for SUN1 antibody 24568-1-APDownload protocol
IP protocol for SUN1 antibody 24568-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols