Tested Applications
| Positive WB detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
Product Information
15000-1-AP targets UNKL in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag6990 Product name: Recombinant human UNKL protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-229 aa of BC000150 Sequence: MTCCSQVPPRRRPSLALSPRLDCNGLNGVPGSIWDFVSGSFSPSPSPILSAGPPSSSSASPNGAELARVRRQLDEAKRKIRQWEESWQQVKQVCDAWQREAQEAKERARVADSDRQLALQKKEEVEAQVKQLQEELEGLGVASTLPGLRGCGDIGTIPLPKLHSLQSQLRLDLEAVDGVIFQLRAKQCVACRERAHGAVLRPCQHHILCEPCAATAPECPYCKGQPLQW Predict reactive species |
| Full Name | unkempt homolog (Drosophila)-like |
| Calculated Molecular Weight | 25 kDa |
| Observed Molecular Weight | 32 kDa, 45 kDa |
| GenBank Accession Number | BC000150 |
| Gene Symbol | UNKL |
| Gene ID (NCBI) | 64718 |
| RRID | AB_2213189 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9H9P5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Putative E3 ubiquitin-protein ligase UNKL was expressed at significantly lower levels in endometrial tumor tissues as compared to the endometrium. Importantly, in human endometrial cancer, primary tumor expression of UNKL was correlated with overall survival in black patients with high mutational burden.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for UNKL antibody 15000-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cancer Biother Radiopharm Tectonic 1 Is a Key Regulator of Cell Proliferation in Pancreatic Cancer. | ||
Toxicol Res (Camb) Celastrol inhibits gastric cancer cell proliferation, migration, and invasion via the FOXA1/CLDN4 axis |
