Filter: AllWBIPIHCIF/ICC

WB analysis using 15547-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 15547-1-AP (UROD antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of rat kidney using 15547-1-AP
rat kidney tissue were subjected to SDS PAGE followed by western blot with 15547-1-AP (UROD Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 15547-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 15547-1-AP (UROD antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 15547-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 15547-1-AP (UROD antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of K-562 using 15547-1-AP
IP result of anti-UROD (IP:15547-1-AP, 4ug; Detection:15547-1-AP 1:500) with K-562 cells lysate 4400 ug.
IHC staining of human kidney using 15547-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15547-1-AP (UROD antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human kidney using 15547-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15547-1-AP (UROD antibody) at dilution of 1:100 (under 40x lens).
IF Staining of HEK-293 using 15547-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using UROD antibody (15547-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

UROD Polyclonal antibody

Name: UROD Polyclonal antibody
Brand: Proteintech
SKU: 15547-1-AP

UROD Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 15547-1-AP

Publications(2)

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

URO-D, EC:4.1.1.37

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Publications (2)
Protocols

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	K-562 cells, HepG2 cells, HEK-293 cells, rat kidney tissue, mouse liver tissue, rat liver tissue
Positive IP detected in	K-562 cells
Positive IHC detected in	human kidney tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HEK-293 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:3000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 2 publications below

Product Information

15547-1-AP targets UROD in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag7905 Product name: Recombinant human UROD protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-367 aa of BC001778 Sequence: MEANGLGPQGFPELKNDTFLRAAWGEETDYTPVWCMRQAGRYLPEFRETRAAQDFFSTCRSPEACCELTLQPLRRFPLDAAIIFSDILVVPQALGMEVTMVPGKGPSFPEPLREEQDLERLRDPEVVASELGYVFQAITLTRQRLAGRVPLIGFAGAPWTLMTYMVEGGGSSTMAQAKRWLYQRPQASHQLLRILTDALVPYLVGQVVAGAQALQLFESHAGHLGPQLFNKFALPYIRDVAKQVKARLREAGLAPVPMIIFAKDGHFALEELAQAGYEVVGLDWTVAPKKARECVGKTVTLQVNLDPCALYASEEEIGQLVKQMLDDFGPHRYIANLGHGLYPDMDPEHVGAFVDAVHKHSRLLRQN Predict reactive species
Full Name	uroporphyrinogen decarboxylase
Calculated Molecular Weight	41 kDa
Observed Molecular Weight	41 kDa
GenBank Accession Number	BC001778
Gene Symbol	UROD
Gene ID (NCBI)	7389
RRID	AB_2213813
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P06132
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for UROD antibody 15547-1-AP	Download protocol
IHC protocol for UROD antibody 15547-1-AP	Download protocol
IF protocol for UROD antibody 15547-1-AP	Download protocol
IP protocol for UROD antibody 15547-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Mol Cell Metabolic-scale gene activation screens identify SLCO2B1 as a heme transporter that enhances cellular iron availability. Authors - Gokhan Unlu View Article
mouse	WB	Cell Mol Gastroenterol Hepatol The histamine pathway is a target to treat hepatic experimental erythropoietic protoporphyria Authors - Ning Kuo View Article