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WB analysis of HeLa using 14354-1-AP
WB result of USP15 antibody (14354-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-USP15 transfected HeLa cells.
WB analysis of mouse brain using 14354-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 14354-1-AP (USP15 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis using 14354-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 14354-1-AP (USP15 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis using 14354-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 14354-1-AP (USP15 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 14354-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 14354-1-AP (USP15 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 14354-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 14354-1-AP (USP15 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of mouse brain using 14354-1-AP
IP result of anti-USP15 (IP:14354-1-AP, 4ug; Detection:14354-1-AP 1:1000) with mouse brain tissue lysate 4000ug.
IHC staining of human breast cancer using 14354-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14354-1-AP (USP15 Antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human breast cancer using 14354-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14354-1-AP (USP15 Antibody) at dilution of 1:200 (under 40x lens).
IHC staining of human placenta using 14354-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 14354-1-AP (USP15 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung using 14354-1-AP
Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 14354-1-AP (USP15 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse kidney using 14354-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 14354-1-AP (USP15 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat small intestine using 14354-1-AP
Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 14354-1-AP (USP15 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human ovary tumor using 14354-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 14354-1-AP (USP15 Antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human ovary tumor using 14354-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 14354-1-AP (USP15 Antibody) at dilution of 1:200 (under 40x lens).

Featured Product
KD/KO Validated

USP15 Polyclonal antibody

USP15 Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 14354-1-AP

Publications(22)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, IP, CoIP, ELISA

Synonyms

Deubiquitinating enzyme 15, EC:3.4.19.12, KIAA0529, Ubiquitin carboxyl-terminal hydrolase 15, Ubiquitin thioesterase 15

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Publications (22)
Protocols
Reviews (1)

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	HEK-293 cells, HeLa cells, mouse brain tissue, HepG2 cells, K-562 cells, MCF-7 cells, NIH/3T3 cells, rat brain tissue
Positive IP detected in	mouse brain tissue
Positive IHC detected in	human placenta tissue, human breast cancer tissue, human lung tissue, human ovary tumor tissue, mouse kidney tissue, rat small intestine tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:12000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 12 publications below
WB	See 19 publications below
IHC	See 2 publications below
IF	See 5 publications below
IP	See 3 publications below
CoIP	See 2 publications below

Product Information

14354-1-AP targets USP15 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, bovine
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag5703 Product name: Recombinant human USP15 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-235 aa of BC020688 Sequence: MAEGGAADLDTQRSDIATLLKTSLRKGDTWYLVDSRWFKQWKKYVGFDSWDKYQMGDQNVYPGPIDNSGLLKDGDAQSLKEHLIDELDYILLPTEGWNKLVSWYTLMEGQEPIARKVVEQGMFVKHCKVEVYLTELKLCENGNMNNVVTRRFSKADTIDTIEKEIRKIFSIPDEKETRLWNKYMSNTFEPLNKPDSTIQDAGLYQGQVLVIEQKNEDGTWPRGPSTPKKPLEQSC Predict reactive species
Full Name	ubiquitin specific peptidase 15
Calculated Molecular Weight	112 kDa
Observed Molecular Weight	112 kDa
GenBank Accession Number	BC020688
Gene Symbol	USP15
Gene ID (NCBI)	9958
RRID	AB_2257148
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9Y4E8
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Ubiquitin is a highly conserved eukaryotic protein that is synthesized as a fusion protein precursor, either to itself or to one of two ribosomal proteins. USP15, is also named as KIAA0529,Unph-2, Unph4, encoding a human ubiquitin-specific protease (USP). The USP15 protein contains the highly conserved Cys and His boxes present in all members of he UBP family of deubiquitinating enzymes(PMID:10444327).It has 3 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
IHC protocol for USP15 antibody 14354-1-AP	Download protocol
IP protocol for USP15 antibody 14354-1-AP	Download protocol
WB protocol for USP15 antibody 14354-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Nat Immunol USP15 stabilizes MDM2 to mediate cancer-cell survival and inhibit antitumor T cell responses. Authors - Qiang Zou KO Validated View Article
mouse,human	WB	Nat Commun ERK and USP5 govern PD-1 homeostasis via deubiquitination to modulate tumor immunotherapy Authors - Xiangling Xiao KD Validated View Article
mouse	WB	Mol Cell RAPIDASH: Tag-free enrichment of ribosome-associated proteins reveals composition dynamics in embryonic tissue, cancer cells, and macrophages Authors - Teodorus Theo Susanto View Article
human	WB	Autophagy USP33 deubiquitinates PRKN/parkin and antagonizes its role in mitophagy. Authors - Kaifeng Niu KD Validated View Article
mouse	WB	Autophagy LncRNA 2810403D21Rik/Mirf promotes ischemic myocardial injury by regulating autophagy through targeting Mir26a. Authors - Haihai Liang View Article
human	WB	Leukemia The deubiquitinase USP15 modulates cellular redox and is a therapeutic target in acute myeloid leukemia Authors - Madeline Niederkorn View Article