Tested Applications
| Positive IHC detected in | mouse brain tissue, human testis tissue, human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells, HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12006-1-AP targets VPS45 in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2658 Product name: Recombinant human VPS45 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 221-570 aa of BC012932 Sequence: DAITPLLNQWTYQAMVHELLGINNNRIDLSRVPGISKDLREVVLSAENDEFYANNMYLNFAEIGSNIKNLMEDFQKKKPKEQQKLESIADMKAFVENYPQFKKMSGTVSKHVTVVGELSRLVSERNLLEVSEVEQELACQNDHSSALQNIKRLLQNPKVTEFDAARLVMLYALHYERHSSNSLPGLMMDLRNKGVSEKYRKLVSAVVEYGGKRVRGSDLFSPKDAVAITKQFLKGLKGVENVYTQHQPFLHETLDHLIKGRLKENLYPYLGPSTLRDRPQDIIVFVIGGATYEEALTVYNLNRTTPGVRIVLGGTTVHNTKSFLEEVLASGLHSRSKESSQVTSRSASRR Predict reactive species |
| Full Name | vacuolar protein sorting 45 homolog (S. cerevisiae) |
| Calculated Molecular Weight | 570 aa, 65 kDa |
| GenBank Accession Number | BC012932 |
| Gene Symbol | VPS45 |
| Gene ID (NCBI) | 11311 |
| RRID | AB_2215369 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NRW7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The Sec1/Munc18 (SM) family of proteins regulate intracellular trafficking through interactions with individual SNARE (soluble N-ethylmeleimide-sensitive factor attachment protein receptor) proteins and assembled SNARE complexes. The SM protein VPS45 (Vacuolar protein sorting-associated protein 45) has a calculated molecular mass of 65 kDa and shares 38% identity with yeast Vps45 which is required for the formation of yeast endosomal SNARE complexes and is thus essential for traffic through the endosomal system. VPS45 may play a role in vesicle-mediated protein trafficking from the Golgi stack through the trans-Golgi network. (PMID: 8996080; 23166732)
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for VPS45 antibody 12006-1-AP | Download protocol |
| IHC protocol for VPS45 antibody 12006-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-VPS45 antibody (Cat# 12006-1-AP) has 5 total citations published in J Gastroenterol, Mol Carcinog, Gene, Nat Commun, and J Hepatocell Carcinoma. Research fields include hepatocellular carcinoma progression, Wnt/β-catenin signaling, Nrf2-mediated antioxidant responses, triple-negative breast cancer metastasis suppression, and tumor immune microenvironment analysis.
| Species | Application | Title |
|---|---|---|
J Gastroenterol VPS45 promotes the progression of hepatocellular carcinoma by recycling β1 integrin to the cell membrane via the endocytic pathway.
| ||
Mol Carcinog VPS45 Contributes to the Progression of Hepatocellular Carcinoma by Triggering the Wnt/β-Catenin Signaling Pathway | ||
Gene Rice protein suppresses ROS generation and stimulates antioxidant gene expression via Nrf2 activation in adult rats. | ||
Nat Commun In vivo CRISPR screening identifies metastasis suppressors in triple-negative breast cancer. | ||
J Hepatocell Carcinoma Prognostic Value of VPS45 in HCC and Its Correlation with Immune Microenvironment. |
Reviews
What customers say
The single review for 12006-1-AP was submitted by Jerome Cattin in May 2020. He used the antibody at a 1:100 dilution on HeLa cells for immunofluorescence and rated it 3 out of 5 stars. His comment described the results as "very inconclusive" with significant background signal, suggesting the antibody may not perform optimally under his experimental conditions for IF applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Jerome (Verified Customer) (05-28-2020) | Very inconclusive, a lot of background.
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