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WB analysis of human stomach using 14145-1-AP
human stomach tissue were subjected to SDS PAGE followed by western blot with 14145-1-AP (VSIG1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of human stomach using 14145-1-AP
human stomach tissue were subjected to SDS PAGE followed by western blot with 14145-1-AP (VSIG1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
IHC staining of human stomach using 14145-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 14145-1-AP (VSIG1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human testis using 14145-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 14145-1-AP (VSIG1 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human testis using 14145-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 14145-1-AP (VSIG1 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human testis using 14145-1-AP
Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 14145-1-AP (VSIG1 Antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human stomach using 14145-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 14145-1-AP (VSIG1 Antibody) at dilution of 1:50 (under 40x lens).

VSIG1 Polyclonal antibody

Cat No. 14145-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

V-set and immunoglobulin domain-containing protein 1, GPA34, Glycoprotein A34, Cell surface A33 antigen

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

Freight/Packing: -

Quantity

- +

Positive WB detected in	human stomach tissue
Positive IHC detected in	human stomach tissue, human testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Application	Dilution
Western Blot (WB)	WB : 1:500-1:3000
Immunohistochemistry (IHC)	IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

WB	See 1 publications below

14145-1-AP targets VSIG1 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag5314 Product name: Recombinant human VSIG1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 256-387 aa of BC043216 Sequence: FARNKAKAKAKERNSKTIAELEPMTKINPRGESEAMPREDATQLEVTLPSSIHETGPDTIQEPDYEPKPTQEPAPEPAPGSEPMAVPDLDIELELEPETQSELEPEPEPEPESEPGVVVEPLSEDEKGVVKA Predict reactive species
Full Name	V-set and immunoglobulin domain containing 1
Calculated Molecular Weight	387 aa, 42 kDa
Observed Molecular Weight	65-70 kDa
GenBank Accession Number	BC043216
Gene Symbol	VSIG1
Gene ID (NCBI)	340547
RRID	AB_2272980
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q86XK7
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Product Specific Protocols
IHC protocol for VSIG1 antibody 14145-1-AP	Download protocol
WB protocol for VSIG1 antibody 14145-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Species	Application	Title
human	WB	Cell Cycle Downregulation of cancer-associated fibroblast exosome-derived miR-29b-1-5p restrains vasculogenic mimicry and apoptosis while accelerating migration and invasion of gastric cancer cells via immunoglobulin domain-containing 1/zonula occluden-1 axis Authors - Chenqu Wu View Article