Tested Applications
| Positive WB detected in | HeLa cells, mouse heart tissue |
| Positive IP detected in | mouse heart tissue |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
10316-1-AP targets WBP11 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag0366 Product name: Recombinant human WBP11 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 170-379 aa of BC001621 Sequence: TSAYGPPTRAVSILPLLGHGVPRLPPGRKPPGPPPGPPPPQVVQMYGRKVGFALDLPPRRRDEDMLYSPELAQRGHDDDVSSTSEDDGYPEDMDQDKHDDSTDDSDTDKSDGESDGDEFVHRDNGERDNNEEKKSGLSVRFADMPGKSRKKKKNMKELTPLQAMMLRMAGQEIPEEGREVEEFSEDDDEDDSDDSEAEKQSQKQHKEESH Predict reactive species |
| Full Name | WW domain binding protein 11 |
| Calculated Molecular Weight | 70 kDa |
| Observed Molecular Weight | 66-70 kDa |
| GenBank Accession Number | BC001621 |
| Gene Symbol | WBP11 |
| Gene ID (NCBI) | 51729 |
| RRID | AB_10694664 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y2W2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
WBP11 (WW domain-binding protein 11) is a protein that in humans, is encoded by the WBP11 gene. The function of WBP11 is to play a role in the regulation of pre-mRNA processing. More specifically, this nuclear protein, colocalizes with mRNA splicing factors and intermediate filament-containing perinuclear networks. The predicted MW of this protein is 70 kDa, and this antibody specially recognises the 70 kDa protein.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for WBP11 antibody 10316-1-AP | Download protocol |
| IHC protocol for WBP11 antibody 10316-1-AP | Download protocol |
| IP protocol for WBP11 antibody 10316-1-AP | Download protocol |
| WB protocol for WBP11 antibody 10316-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
