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WDR40A Polyclonal antibody

WDR40A Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 20478-1-AP

Synonyms

DCAF12, KIAA1892, TCC52, WD repeat domain 40A, WDR40A

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using WDR40A Polyclonal antibody (20478-1-AP)

    WB analysis of HEK-293 using 20478-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 20478-1-AP (WDR40A Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using WDR40A Polyclonal antibody (20478-1-AP)

    WB analysis of Jurkat using 20478-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 20478-1-AP (WDR40A Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human testis tissue using WDR40A Polyclonal antibody (20478-1-AP)

    IHC staining of human testis using 20478-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 20478-1-AP (WDR40A Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using WDR40A Polyclonal antibody (20478-1-AP)

    IHC staining of human testis using 20478-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 20478-1-AP (WDR40A Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using WDR40A Polyclonal antibody (20478-1-AP)

    IHC staining of human lung cancer using 20478-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 20478-1-AP (WDR40A Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using WDR40A Polyclonal antibody (20478-1-AP)

    IHC staining of human lung cancer using 20478-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 20478-1-AP (WDR40A Antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inHEK-293 cells, Jurkat cells
Positive IHC detected inhuman testis tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 20478-1-AP is WDR40A Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen WDR40A fusion protein Ag14349
Full Name WD repeat domain 40A
Calculated Molecular Weight 453 aa, 51 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC008893
Gene Symbol WDR40A
Gene ID (NCBI) 25853
RRIDAB_2878692
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

WDR40A, also known as DCAF12, is a 51 kDa protein. It may function as a substrate receptor for CUL4-DDB1 E3 ubiquitin-protein ligase complex(PMID: 16949367). This antibody specifically recognises the 51 kDa protein.

Protocols

Product Specific Protocols
WB protocol for WDR40A antibody 20478-1-APDownload protocol
IHC protocol for WDR40A antibody 20478-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols