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  • Western Blot (WB) analysis of HEK-293 cells using WDR40A Polyclonal antibody (20478-1-AP)

    WB analysis of HEK-293 using 20478-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 20478-1-AP (WDR40A Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using WDR40A Polyclonal antibody (20478-1-AP)

    WB analysis of Jurkat using 20478-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 20478-1-AP (WDR40A Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using WDR40A Polyclonal antibody (20478-1-AP)

    IHC staining of human lung cancer using 20478-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 20478-1-AP (WDR40A antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human thyroid cancer tissue using WDR40A Polyclonal antibody (20478-1-AP)

    IHC staining of human thyroid cancer using 20478-1-AP

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 20478-1-AP (WDR40A antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using WDR40A Polyclonal antibody (20478-1-AP)

    IHC staining of human lung cancer using 20478-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 20478-1-AP (WDR40A Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using WDR40A Polyclonal antibody (20478-1-AP)

    IHC staining of human lung cancer using 20478-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 20478-1-AP (WDR40A Antibody) at dilution of 1:50 (under 40x lens).

WDR40A Polyclonal antibody

WDR40A Polyclonal Antibody for WB, IHC, ELISA
Cat No. 20478-1-AP

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

Testis cancer centrosome-related protein, TCC52, KIAA1892, DDB1- and CUL4-associated factor 12, DCAF12

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive WB detected inHEK-293 cells, Jurkat cells
Positive IHC detected inhuman lung cancer tissue, human thyroid cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20478-1-AP targets WDR40A in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag14349

Product name: Recombinant human WDR40A protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-56 aa of BC008893

Sequence: LHKRKRLPPVKRSLVYYLKNREVRLQNETSYSRVLHGYAAQQLPSLLKEREFHLGT

Predict reactive species
Full Name WD repeat domain 40A
Calculated Molecular Weight 453 aa, 51 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC008893
Gene Symbol WDR40A
Gene ID (NCBI) 25853
RRIDAB_2878692
Conjugate Unconjugated
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ5T6F0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

WDR40A, also known as DCAF12, is a 51 kDa protein. It may function as a substrate receptor for CUL4-DDB1 E3 ubiquitin-protein ligase complex(PMID: 16949367). This antibody specifically recognises the 51 kDa protein.

Protocols

Product Specific Protocols
IHC protocol for WDR40A antibody 20478-1-APDownload protocol
WB protocol for WDR40A antibody 20478-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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