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WHSC1 Polyclonal antibody

WHSC1 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 22722-1-AP

Synonyms

KIAA1090, MMSET, NSD2, Protein trithorax 5, REIIBP, TRX5, WHS, WHSC1

Validation Data Gallery

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  • Western Blot (WB) analysis of HCT 116 cells using WHSC1 Polyclonal antibody (22722-1-AP)

    WB analysis of HCT 116 using 22722-1-AP

    HCT 116 cells were subjected to SDS PAGE followed by western blot with 22722-1-AP (WHSC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using WHSC1 Polyclonal antibody (22722-1-AP)

    IP experiment of HeLa using 22722-1-AP

    IP result of anti-WHSC1 (IP:22722-1-AP, 3ug; Detection:22722-1-AP 1:300) with HeLa cells lysate 2000ug.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using WHSC1 Polyclonal antibody (22722-1-AP)

    IHC staining of human breast cancer using 22722-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22722-1-AP (WHSC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using WHSC1 Polyclonal antibody (22722-1-AP)

    IHC staining of human breast cancer using 22722-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22722-1-AP (WHSC1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using WHSC1 Polyclonal antibody (22722-1-AP)

    IHC staining of human breast cancer using 22722-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22722-1-AP (WHSC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHCT 116 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

22722-1-AP targets WHSC1 in WB, IP, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen WHSC1 fusion protein Ag18611
Full Name Wolf-Hirschhorn syndrome candidate 1
Calculated Molecular Weight 162 kDa
Observed Molecular Weight 70 kDa, 150 kDa
GenBank Accession NumberBC152412
Gene Symbol WHSC1
Gene ID (NCBI) 7468
RRIDAB_2879156
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

WHSC1 (also known as MMSET or NSD2) is a SET domain containing histone lysine methyltransferase that can di- and trimethylate histone H3 at lysine 36 (H3K36). WHSC1 mRNA is highly expressed in embryonic tissues, especially those with high degrees of proliferation, whereas in adult tissues,the mRNA is primarily expressed in thymus and testis. WHSC1 is implicated in cellular adhesion, clonogenic growth and tumorigenicity. Its overexpression has been found in various cancers. It undergoes alternative splicing resulting in different protein isoforms. Recently WHSC1 has been identified as a critical epigenetic regulator for Tfh differentiation.

Protocols

Product Specific Protocols
WB protocol for WHSC1 antibody 22722-1-APDownload protocol
IHC protocol for WHSC1 antibody 22722-1-APDownload protocol
IP protocol for WHSC1 antibody 22722-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols