WIPF2 Polyclonal antibody

WIPF2 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 20512-1-AP

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

PP10631, WAS/WASL-interacting protein family member 2, WASP-interacting protein-related protein, WICH, WIP- and CR16-homologous protein

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

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Filter: AllWBIHC

WB analysis of mouse brain using 20512-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 20512-1-AP (WIPF2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of mouse colon using 20512-1-AP
mouse colon tissue were subjected to SDS PAGE followed by western blot with 20512-1-AP (WIPF2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human breast cancer using 20512-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20512-1-AP (WIPF2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human placenta using 20512-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 20512-1-AP (WIPF2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human rectal cancer using 20512-1-AP
Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 20512-1-AP (WIPF2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse kidney using 20512-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 20512-1-AP (WIPF2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of rat kidney using 20512-1-AP
Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 20512-1-AP (WIPF2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human liver cancer using 20512-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20512-1-AP (WIPF2 antibody) at dilution of 1:200 (under 0x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Overview

Tested Applications
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MSDS SDS

Tested Applications

Positive WB detected in	mouse brain tissue, mouse colon tissue
Positive IHC detected in	human breast cancer tissue, human liver cancer tissue, mouse kidney tissue, rat kidney tissue, human placenta tissue, human rectal cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20512-1-AP targets WIPF2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag14330 Product name: Recombinant human WIPF2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 18-176 aa of BC065551 Sequence: TFHQANTEQPKLSRDEQRGRGALLQDICKGTKLKKVTNINDRSAPILEKPKGSSGGYGSGGAALQPKGGLFQGGVLKLRPVGAKDGSENLAGKPALQIPSSRAAAPRPPVSAASGRPQDDTDSSRASLPELPRMQRPSLPDLSRPNTTSSTGMKHSSSA Predict reactive species
Full Name	WAS/WASL interacting protein family, member 2
Calculated Molecular Weight	440 aa, 46 kDa
Observed Molecular Weight	46-50 kDa
GenBank Accession Number	BC065551
Gene Symbol	WIPF2
Gene ID (NCBI)	147179
RRID	AB_10695185
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q8TF74
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.