- Featured Product
- KD/KO Validated
XAB2 Polyclonal antibody
XAB2 Polyclonal Antibody for IP, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IP, IF, ELISA
Conjugate
Unconjugated
Cat no : 10637-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | K-562 cells, HeLa cells |
| Positive IP detected in | K-562 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 3 publications below |
| IF | See 1 publications below |
Product Information
10637-1-AP targets XAB2 in WB, IP, IF, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | XAB2 fusion protein Ag0999 |
| Full Name | XPA binding protein 2 |
| Calculated Molecular Weight | 100 kDa |
| Observed Molecular Weight | 100 kDa |
| GenBank Accession Number | BC007208 |
| Gene Symbol | XAB2 |
| Gene ID (NCBI) | 56949 |
| RRID | AB_2257336 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
XPA-binding protein 2 (XAB2) a tetratricopeptide repeat protein involved in transcription-coupled DNA repair and transcription. XAB2 is involved in pre-mRNA splicing, transcription, and transcription-coupled DNA repair, leading to preimplantation lethality, and is essential for mouse embryogenesis. It interactes with Cockayne syndrome groups A and B (CSA and CSB) proteins and RNA polymerase II, which are known to be involved in transcription-coupled repair (TCR) and transcription, and that the antibodies against XAB2 protein inhibited the recovery of RNA synthesis after UV irradiation and normal RNA synthesis when microinjected into living fibroblasts.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for XAB2 antibody 10637-1-AP | Download protocol |
| IP protocol for XAB2 antibody 10637-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nucleic Acids Res XAB2 depletion induces intron retention in POLR2A to impair global transcription and promote cellular senescence.
| ||
Nucleic Acids Res Evidence that a consensus element found in naturally intronless mRNAs promotes mRNA export.
| ||
Cell Death Dis XAB2 functions in mitotic cell cycle progression via transcriptional regulation of CENPE.
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