ZIC3 Polyclonal antibody

ZIC3 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 21717-1-AP

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

ZNF203, Zinc finger protein ZIC 3

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

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Filter: AllWBIHC

WB analysis of Neuro-2a using 21717-1-AP
Neuro-2a cells were subjected to SDS PAGE followed by western blot with 21717-1-AP (ZIC3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of SH-SY5Y using 21717-1-AP
SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 21717-1-AP (ZIC3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of SH-SY5Y using 21717-1-AP
SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 21717-1-AP (ZIC3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of mouse cerebellum using 21717-1-AP
mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 21717-1-AP (ZIC3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of Y79 using 21717-1-AP
Y79 cells were subjected to SDS PAGE followed by western blot with 21717-1-AP (ZIC3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of SH-SY5Y using 21717-1-AP
SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 21717-1-AP (ZIC3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of mouse cerebellum using 21717-1-AP
mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 21717-1-AP (ZIC3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse brain using 21717-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21717-1-AP (ZIC3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of rat brain using 21717-1-AP
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 21717-1-AP (ZIC3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of rat cerebellum using 21717-1-AP
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 21717-1-AP (ZIC3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human urothelial carcinoma using 21717-1-AP
Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 21717-1-AP (ZIC3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse cerebellum using 21717-1-AP
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 21717-1-AP (ZIC3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human thyroid cancer using 21717-1-AP
Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 21717-1-AP (ZIC3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Overview

Tested Applications
Product Information
Protocols
Reviews (1)

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MSDS SDS

Tested Applications

Positive WB detected in	Neuro-2a cells, mouse cerebellum tissue, SH-SY5Y cells, Y79 cells
Positive IHC detected in	human urothelial carcinoma tissue, human thyroid cancer tissue, mouse brain tissue, mouse cerebellum tissue, rat brain tissue, rat cerebellum tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunohistochemistry (IHC)	IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21717-1-AP targets ZIC3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag16354 Product name: Recombinant human ZIC3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-200 aa of BC113395 Sequence: MTMLLDGGPQFPGLGVGSFGAPRHHEMPNREPAGMGLNPFGDSTHAAAAAAAAAAFKLSPAAAHDLSSGQSSAFTPQGSGYANALGHHHHHHHHHHHTSQVPSYGGAASAAFNSTREFLFRQRSSGLSEAASGGGQHGLFAGSASSLHAPAGIPEPPSYLLFPGLHEQGAGHPSPTGHVDNNQVHLGLRGELFGRADPYR Predict reactive species
Full Name	Zic family member 3 (odd-paired homolog, Drosophila)
Calculated Molecular Weight	467 aa, 51 kDa
Observed Molecular Weight	45-50 kDa
GenBank Accession Number	BC113395
Gene Symbol	ZIC3
Gene ID (NCBI)	7547
RRID	AB_2878910
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	O60481
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.