ZNF146 Polyclonal antibody

ZNF146 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 20385-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ChIP, ELISA

Synonyms

Only zinc finger protein, OZF, zinc finger protein 146, Zinc finger protein OZF, ZNF146

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

SKU:

Freight/Packing: -

Quantity

- +

Filter: AllWBIHC

WB analysis of mouse kidney using 20385-1-AP
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 20385-1-AP (ZNF146 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of mouse lung using 20385-1-AP
mouse lung tissue were subjected to SDS PAGE followed by western blot with 20385-1-AP (ZNF146 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human heart using 20385-1-AP
Immunohistochemical analysis of paraffin-embedded human heart using 20385-1-AP (ZNF146 antibody) at dilution of 1:100 (under 10x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human heart using 20385-1-AP
Immunohistochemical analysis of paraffin-embedded human heart using 20385-1-AP (ZNF146 antibody) at dilution of 1:100 (under 40x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Positive WB detected in	mouse kidney tissue, mouse lung tissue
Positive IHC detected in	human heart tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

20385-1-AP targets ZNF146 in WB, IHC, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag14223 Product name: Recombinant human ZNF146 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 44-292 aa of BC005154 Sequence: FECNECGKAFSQKQYVIKHQNTHTGEKLFECNECGKSFSQKENLLTHQKIHTGEKPFECKDCGKAFIQKSNLIRHQRTHTGEKPFVCKECGKTFSGKSNLTEHEKIHIGEKPFKCSECGTAFGQKKYLIKHQNIHTGEKPYECNECGKAFSQRTSLIVHVRIHSGDKPYECNVCGKAFSQSSSLTVHVRSHTGEKPYGCNECGKAFSQFSTLALHLRIHTGKKPYQCSECGKAFSQKSHHIRHQKIHTH Predict reactive species
Full Name	zinc finger protein 146
Calculated Molecular Weight	292 aa, 33 kDa
Observed Molecular Weight	20-25 kDa, 33-35 kDa
GenBank Accession Number	BC005154
Gene Symbol	ZNF146
Gene ID (NCBI)	7705
RRID	AB_10694154
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q15072
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Product Specific Protocols
IHC protocol for ZNF146 antibody 20385-1-AP	Download protocol
WB protocol for ZNF146 antibody 20385-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Species	Application	Title
human	ChIP	Reproduction ZNF146 regulates cell cycle progression via TFDP1 and DEPDC1B in ovarian cancer cells Authors - Ruixue Zhao View Article
human,mouse	WB,IHC	J Gastrointest Oncol Hsa_circ_NOTCH3 regulates ZNF146 through sponge adsorption of miR-875-5p to promote tumorigenesis of hepatocellular carcinoma. Authors - Lei Bao View Article
human	WB,IHC,ChIP	Cell Biosci ZNF146 accelerates lung adenocarcinoma progression through MDM2/p53 and PHGDH/ferroptosis. Authors - Junkan Zhu KO Validated View Article