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ZNF385A Polyclonal antibody

ZNF385A Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 26288-1-AP

Synonyms

HZF, Retinal zinc finger protein, RZF, ZFP385, zinc finger protein 385A, ZNF385, ZNF385A

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using ZNF385A Polyclonal antibody (26288-1-AP)

    WB analysis of HeLa using 26288-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 26288-1-AP (ZNF385A Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ZNF385A Polyclonal antibody (26288-1-AP)

    IF Staining of HeLa using 26288-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 26288-1-AP (ZNF385A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 26288-1-AP is ZNF385A Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ZNF385A fusion protein Ag23577
Full Name zinc finger protein 385A
Observed Molecular Weight 45-50 kDa
GenBank Accession NumberBC029752
Gene Symbol ZNF385A
Gene ID (NCBI) 25946
RRIDAB_2880464
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for ZNF385A antibody 26288-1-APDownload protocol
IF protocol for ZNF385A antibody 26288-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Int J Mol Sci

ZNF385A and ZNF346 Serve as Prognostic Biomarkers Associated with an Inflamed Immunosuppressive Tumor Microenvironment in Hepatocellular Carcinoma

Authors - Qihang Peng
  • KD Validated
View Article