ZNF394 Polyclonal antibody

ZNF394 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 25785-1-AP

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

ZKSCAN14

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

SKU:

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Quantity

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Filter: AllWBIHC

WB analysis of mouse brain using 25785-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 25785-1-AP (ZNF394 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of fetal human brain using 25785-1-AP
fetal human brain tissue were subjected to SDS PAGE followed by western blot with 25785-1-AP (ZNF394 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of mouse spleen using 25785-1-AP
mouse spleen tissue were subjected to SDS PAGE followed by western blot with 25785-1-AP (ZNF394 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of HepG2 using 25785-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 25785-1-AP (ZNF394 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human urothelial carcinoma using 25785-1-AP
Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 25785-1-AP (ZNF394 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Overview

Tested Applications
Product Information
Protocols
Reviews (1)

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MSDS SDS

Tested Applications

Positive WB detected in	mouse brain tissue, fetal human brain tissue, HepG2 cells, mouse spleen tissue
Positive IHC detected in	human urothelial carcinoma tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25785-1-AP targets ZNF394 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag22655 Product name: Recombinant human ZNF394 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 140-375 aa of BC051847 Sequence: RALQRALDGTSSQGMVTFEDTAVSLTWEEWERLDPARRDFCRESAQKDSGSTVPPSLESRVENKELIPMQQILEEAEPQGQLQEAFQGKRPLFSKCGSTHEDRVEKQSGDPLPLKLENSPEAEGLNSISDVNKNGSIEGEDSKNNELQNSARCSNLVLCQHIPKAERPTDSEEHGNKCKQSFHMVTWHVLKPHKSDSGDSFHHSSLFETQRQLHEERPYKCGNCGKSFKQRSDLFR Predict reactive species
Full Name	zinc finger protein 394
Observed Molecular Weight	50-60 kDa
GenBank Accession Number	BC051847
Gene Symbol	ZNF394
Gene ID (NCBI)	84124
RRID	AB_2880239
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q53GI3
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ZNF394 (zinc finger protein 394) is a C2H2-type zinc finger protein belonging to the Krüppel C2H2-type zinc finger protein family. The protein contains three distinct domains: the N-terminus has two domains, LER and KRAB, and the C-terminus has seven C2H2 zinc finger domains. ZNF394 is predicted to have DNA-binding transcription factor activity and is involved in the transcriptional regulation of RNA polymerase II. Additionally, ZNF394, together with ZIM3, plays a key role in human embryonic development at the 8-cell stage by inhibiting the expression of genes specific to the 4-cell stage, thereby promoting embryonic development.