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WB analysis of HeLa using 25228-1-AP
WB result of ZNF451 antibody (25228-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-ZNF451 transfected HeLa cells.
WB analysis of HeLa using 25228-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 25228-1-AP (ZNF451 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 25228-1-AP
IP result of anti-ZNF451 (IP:25228-1-AP, 4ug; Detection:25228-1-AP 1:300) with HeLa cells lysate 1080 ug.
IP experiment of HeLa using 25228-1-AP
IP result of anti-ZNF451 (IP:25228-1-AP, 4ug; Detection:25228-1-AP 1:300) with HeLa cells lysate 4000ug.
IP experiment of HeLa using 25228-1-AP
IP result of anti-ZNF451 (IP:25228-1-AP, 4ug; Detection:25228-1-AP 1:300) with HeLa cells lysate 1080 ug.
IHC staining of human placenta using 25228-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 25228-1-AP (ZNF451 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human placenta using 25228-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 25228-1-AP (ZNF451 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 25228-1-AP
Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 25228-1-AP (ZNF451 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Featured Product
KD/KO Validated

ZNF451 Polyclonal antibody

Name: ZNF451 Polyclonal antibody
Brand: Proteintech
SKU: 25228-1-AP

ZNF451 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 25228-1-AP

Publications(4)

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

KIAA1702, KIAA0576, EC:2.3.2.-, E3 SUMO-protein transferase ZNF451, E3 SUMO-protein ligase ZNF451

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Publications (4)
Protocols

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	HeLa cells
Positive IP detected in	HeLa cells
Positive IHC detected in	human placenta tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:200-1:1000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 4 publications below
IHC	See 1 publications below
IF	See 1 publications below

Product Information

25228-1-AP targets ZNF451 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag18346 Product name: Recombinant human ZNF451 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-350 aa of BC021712 Sequence: MGDPGSEIIESVPPAGPEASESTTDENEDDIQFVSEGPLRPVLEYIDLVSSDDEEPSTSYTDENIKRKDHIDYQKDKVALTLARLARHVEVEKQQKEEKNRAFREKIDFQHAHGLQELEFIRGHSDTEAARLCVDQWLKMPGLKTGTINCGTKSSFRRGGHTWVSGKPILCPIMHCNKEFDNGHLLLGHLKRFDHSPCDPTITLHGPFFSSFACVVCYKKFVTQQQYRDHLFDKEATDDGHNNNLLPQIIQCFACPNCFLLFSRKEECSKHMSGKNHFHQSFKLGDNKGIAHPISFPSFAKKLLISLCKDVPFQVKCVACHKTLRSHMELTAHFRVHCRNAGPVAVAEKS Predict reactive species
Full Name	zinc finger protein 451
Calculated Molecular Weight	1061 aa, 121 kDa
Observed Molecular Weight	121 kDa
GenBank Accession Number	BC021712
Gene Symbol	ZNF451
Gene ID (NCBI)	26036
RRID	AB_2879975
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9Y4E5
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IF protocol for ZNF451 antibody 25228-1-AP	Download protocol
IHC protocol for ZNF451 antibody 25228-1-AP	Download protocol
IP protocol for ZNF451 antibody 25228-1-AP	Download protocol
WB protocol for ZNF451 antibody 25228-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	PLoS Pathog Changes in SUMO-modified proteins in Epstein-Barr virus infection identifies reciprocal regulation of TRIM24/28/33 complexes and the lytic switch BZLF1 Authors - Carlos F De La Cruz-Herrera View Article
human	WB	Research (Wash D C) Single-Cell Spatial-Temporal Analysis of ZNF451 in Mediating Drug Resistance and CD8+ T Cell Dysfunction Authors - Ning Tang View Article
human	WB,IHC,IF	Cell Rep ZNF451 favors triple-negative breast cancer progression by enhancing SLUG-mediated CCL5 transcriptional expression Authors - Yu Zhang KD Validated View Article
mouse	WB	Respir Res Loss of ZNF451 mediates fibroblast activation and promotes lung fibrosis Authors - Hong Peng View Article