Tested Applications
| Positive WB detected in | HeLa cells |
| Positive IP detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
25817-1-AP targets ZNF639 in WB, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag19854 Product name: Recombinant human ZNF639 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 136-485 aa of BC026181 Sequence: EDVPIAVEVHAISEDYDIETENNSSESLQDQTDEEPPAKLCKILDKSQALNVTAQQKWPLLRANSSGLYKCELCEFNSKYFSDLKQHMILKHKRTDSNVCRVCKESFSTNMLLIEHAKLHEEDPYICKYCDYKTVIFENLSQHIADTHFSDHLYWCEQCDVQFSSSSELYLHFQEHSCDEQYLCQFCEHETNDPEDLHSHVVNEHACKLIELSDKYNNGEHGQYSLLSKITFDKCKNFFVCQVCGFRSRLHTNVNRHVAIEHTKIFPHVCDDCGKGFSSMLEYCKHLNSHLSEGIYLCQYCEYSTGQIEDLKIHLDFKHSADLPHKCSDCLMRFGNERELISHLPVHETT Predict reactive species |
| Full Name | zinc finger protein 639 |
| Calculated Molecular Weight | 485 aa, 56 kDa |
| Observed Molecular Weight | 65-70 kDa |
| GenBank Accession Number | BC026181 |
| Gene Symbol | ZNF639 |
| Gene ID (NCBI) | 51193 |
| RRID | AB_2880251 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UID6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ZNF639, also named as ZASC1, is a 485 amino acid protein, which locates in the nucleus and contains 8 C2H2-type zinc fingers and belongs to the krueppel C2H2-type zinc-finger protein family. ZNF639 binds DNA and may function as a transcriptional repressor.
Protocols
| Product Specific Protocols | |
|---|---|
| IP protocol for ZNF639 antibody 25817-1-AP | Download protocol |
| WB protocol for ZNF639 antibody 25817-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
