Tested Applications
| Positive WB detected in | HeLa cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
25817-1-AP targets ZNF639 in WB, IHC, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag19854 Product name: Recombinant human ZNF639 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 136-485 aa of BC026181 Sequence: EDVPIAVEVHAISEDYDIETENNSSESLQDQTDEEPPAKLCKILDKSQALNVTAQQKWPLLRANSSGLYKCELCEFNSKYFSDLKQHMILKHKRTDSNVCRVCKESFSTNMLLIEHAKLHEEDPYICKYCDYKTVIFENLSQHIADTHFSDHLYWCEQCDVQFSSSSELYLHFQEHSCDEQYLCQFCEHETNDPEDLHSHVVNEHACKLIELSDKYNNGEHGQYSLLSKITFDKCKNFFVCQVCGFRSRLHTNVNRHVAIEHTKIFPHVCDDCGKGFSSMLEYCKHLNSHLSEGIYLCQYCEYSTGQIEDLKIHLDFKHSADLPHKCSDCLMRFGNERELISHLPVHETT Predict reactive species |
| Full Name | zinc finger protein 639 |
| Calculated Molecular Weight | 485 aa, 56 kDa |
| Observed Molecular Weight | 65-70 kDa |
| GenBank Accession Number | BC026181 |
| Gene Symbol | ZNF639 |
| Gene ID (NCBI) | 51193 |
| RRID | AB_2880251 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UID6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ZNF639, also named as ZASC1, is a 485 amino acid protein, which locates in the nucleus and contains 8 C2H2-type zinc fingers and belongs to the krueppel C2H2-type zinc-finger protein family. ZNF639 binds DNA and may function as a transcriptional repressor.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for ZNF639 antibody 25817-1-AP | Download protocol |
| IP protocol for ZNF639 antibody 25817-1-AP | Download protocol |
| WB protocol for ZNF639 antibody 25817-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Reviews
What customers say
One reviewer (Zhannan Han) rated this antibody 5/5 stars for Western Blot on 293T overexpression cells at a 1:1000 dilution. They reported a strong, clean signal with minimal background and the band appearing at the expected molecular weight, describing the antibody as reliable and highly effective in their assays.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Zhannan (Verified Customer) (12-04-2025) | The primary antibody performs excellently. It delivers a strong and clean signal with minimal background, and the band appears precisely at the expected molecular weight. Overall, it has been very reliable and highly effective in our assays.
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