Tested Applications
| Positive WB detected in | human testis tissue |
| Positive IHC detected in | human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
25600-1-AP targets ZNF645 in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag22346 Product name: Recombinant human ZNF645 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 287-425 aa of BC126190 Sequence: SPSSPVNHQMPYPPQDVVTPNSVRSQVPALTTTYDPSSGYIIVKVPPDMNSPPLRAPQSQNGNPSASEFASHHYNLNILPQFTENQETLSPQFTQTDAMDHRRWPAWKRLSPCPPTRSPPPSTLHGRSHHSHQRRHRRY Predict reactive species |
| Full Name | zinc finger protein 645 |
| Calculated Molecular Weight | 425 aa, 49 kDa |
| Observed Molecular Weight | 49 kDa |
| GenBank Accession Number | BC126190 |
| Gene Symbol | ZNF645 |
| Gene ID (NCBI) | 158506 |
| RRID | AB_2880153 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8N7E2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for ZNF645 antibody 25600-1-AP | Download protocol |
| WB protocol for ZNF645 antibody 25600-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Reviews
What customers say
One reviewer (May 2023) reported using this antibody at a 1:1000 dilution for Western Blot on human white adipocytes. They observed two bands, with the lower band at the predicted molecular weight, and rated the product 3 out of 5 stars. Overall, the review suggests the antibody performs as expected in its intended application.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Mi (Verified Customer) (05-29-2023) | It works well in human white adipocytes, we get two bands, with the lower band at the predicted size.
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